Rapid, Label-Free Prediction of Antibiotic Resistance in Salmonella typhimurium by Surface-Enhanced Raman Spectroscopy
In: International Journal of Molecular Sciences, Jg. 23 (2022), Heft 1356
Online
unknown
Zugriff:
The rapid identification of bacterial antibiotic susceptibility is pivotal to the rational administration of antibacterial drugs. In this study, cefotaxime (CTX)-derived resistance in Salmonella typhimurium (abbr. CTXr-S. typhimurium) during 3 months of exposure was rapidly recorded using a portable Raman spectrometer. The molecular changes that occurred in the drug-resistant strains were sensitively monitored in whole cells by label-free surface-enhanced Raman scattering (SERS). Various degrees of resistant strains could be accurately discriminated by applying multivariate statistical analyses to bacterial SERS profiles. Minimum inhibitory concentration (MIC) values showed a positive linear correlation with the relative Raman intensities of I990/I1348, and the R2 reached 0.9962. The SERS results were consistent with the data obtained by MIC assays, mutant prevention concentration (MPC) determinations, and Kirby-Bauer antibiotic susceptibility tests (K-B tests). This preliminary proof-of-concept study indicates the high potential of the SERS method to supplement the time-consuming conventional method and help alleviate the challenges of antibiotic resistance in clinical therapy.
Titel: |
Rapid, Label-Free Prediction of Antibiotic Resistance in Salmonella typhimurium by Surface-Enhanced Raman Spectroscopy
|
---|---|
Autor/in / Beteiligte Person: | Zhang, Ping ; Wu, Xi-Hao ; Su, Lan ; Wang, Hui-Qin ; Lin, Tai-Feng ; Fang, Ya-Ping ; Zhao, Hui-Min ; Lu, Wen-Jing ; Liu, Meng-Jia ; Liu, Wen-Bo ; Zheng, Da-Wei |
Link: | |
Zeitschrift: | International Journal of Molecular Sciences, Jg. 23 (2022), Heft 1356 |
Veröffentlichung: | MDPI AG, 2022 |
Medientyp: | unknown |
ISSN: | 1661-6596 (print) ; 1422-0067 (print) |
Schlagwort: |
|
Sonstiges: |
|