Presence of mrr- and mcr-like restriction systems in coryneform bacteria
In: Research in microbiology, Jg. 144 (1993-03-01), Heft 3
Online
unknown
Zugriff:
Efficient transformation of Brevibacterium flavum MJ233C and Corynebacterium glutamicum ATCC 31831 (up to 5.0 x 10(7) transformants/microgram DNA) depends on the source of plasmid DNA. The transformation efficiencies of B. flavum MJ233C and C. glutamicum ATCC 31831 increased nearly 10(3)-fold when plasmid DNA was isolated from the recipient strain itself or from a damdcm Escherichia coli mutant, as compared with DNA passed through a modification-proficient E. coli strain. These results suggest the presence of a methyl-specific restriction system in certain strains of coryneform bacteria. In addition, electroporation conditions were optimized.
Titel: |
Presence of mrr- and mcr-like restriction systems in coryneform bacteria
|
---|---|
Autor/in / Beteiligte Person: | Inui, Masayuki ; Kurusu, Yasurou ; Yukawa, Hideaki ; Vertès, Alain A. ; Kobayashi, Miki |
Link: | |
Zeitschrift: | Research in microbiology, Jg. 144 (1993-03-01), Heft 3 |
Veröffentlichung: | 1993 |
Medientyp: | unknown |
ISSN: | 0923-2508 (print) |
Schlagwort: |
|
Sonstiges: |
|