High-level expression of a sika deer (Cervus nippon) Cu/Zn superoxide dismutase in Pichia pastoris and its characterization
In: Environmental Toxicology and Pharmacology, Jg. 35 (2013-03-01), S. 185-192
Online
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Zugriff:
Production of a sika deer Cu/Zn-SOD was achieved in Pichia pastoris after the reconstituted expression vector pPIC9K was transformed into the strain GS115. By employing Saccharomyces cerevisiae secretion signal peptide (α-factor) under the regulation of the methanol-inducible promoter of the gene of alcohol oxidase 1 (AOX1), sika deer Cu/Zn-SOD with a molecular mass of 16 kDa was expressed while recombinant sika deer Cu/Zn-SOD with an activity of 3500 U/mL was obtained from a 5 L bioreactor. After two successive steps of chromatography on DEAE-650 C and Superdex75, recombinant sika deer Cu/Zn-SOD was obtained with 13.8% yield, 14.5-fold purification, and a specific activity of 3447 U/mg. Its optimum temperature and optimum pH were 40 °C and 7.0, respectively.
| Titel: |
High-level expression of a sika deer (Cervus nippon) Cu/Zn superoxide dismutase in Pichia pastoris and its characterization
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| Autor/in / Beteiligte Person: | Li, Ren-Kuan ; Fu, Cai-Li ; Chen, Ping ; Ye, Xiuyun ; Tzi Bun Ng |
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| Zeitschrift: | Environmental Toxicology and Pharmacology, Jg. 35 (2013-03-01), S. 185-192 |
| Veröffentlichung: | Elsevier BV, 2013 |
| Medientyp: | unknown |
| ISSN: | 1382-6689 (print) |
| DOI: | 10.1016/j.etap.2012.11.013 |
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