Tumor Necrosis Factor‐a Stimulates Colony Formation by a Megakaryoblastic Leukemia Cell Line, CMK
In: Japanese Journal of Cancer Research : Gann, Jg. 83 (1992), Heft 9, S. 989-994
Online
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Zugriff:
The CMK cell line is an acute megakaryoblastic leukemia cell line established from a patient with Down's syndrome, and is known to possess characteristics of normal megakaryocytes. Several cytokines with the ability to stimulate megakaryopoiesis, such as interleukin-3 (IL-3), interleukin-6 (IL-6) and granulocyte-macrophage colony-stimulating factor (GM-CSF), stimulated colony formation by CMK cells. The present study revealed that tumor necrosis factor-alpha (TNF-alpha) stimulated colony formation by CMK cells; the potency was almost equal to that of IL-3, IL-6 or GM-CSF. Scatchard plot analysis revealed that CMK cells possess two types of specific binding sites for TNF-alpha. The high-affinity binding sites had an affinity constant of 0.18 nM, and numbered 5,000. The low-affinity binding sites had an affinity constant of 1.8 nM and numbered 19,000. These results raise the possibility that TNF-alpha can act as a growth-stimulating agent on megakaryocyte-lineage cell line.
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Tumor Necrosis Factor‐a Stimulates Colony Formation by a Megakaryoblastic Leukemia Cell Line, CMK
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Autor/in / Beteiligte Person: | Sato, Takeyuki ; Yamaguchi, Ken ; Abe, Kaoru ; Akiyama, Yasuto |
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Zeitschrift: | Japanese Journal of Cancer Research : Gann, Jg. 83 (1992), Heft 9, S. 989-994 |
Veröffentlichung: | Blackwell Publishing Ltd, 1992 |
Medientyp: | unknown |
ISSN: | 1876-4673 (print) ; 0910-5050 (print) |
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