CD4+ T cell responses to the Plasmodium falciparum erythrocyte membrane protein 1 in children with mild malaria
In: Journal of immunology (Baltimore, Md. : 1950), Jg. 192 (2014-02-01), Heft 4
Online
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Zugriff:
The immune response against the variant surface Ag Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is a key component of clinical immunity against malaria. We have investigated the development and maintenance of CD4+ T cell responses to a small semiconserved area of the Duffy binding–like domain (DBL)α–domain of PfEMP1, the DBLα-tag. Young children were followed up longitudinally, and parasites and PBMCs were isolated from 35 patients presenting with an acute case of uncomplicated malaria. The DBLα-tag from the PfEMP1 dominantly expressed by the homologous parasite isolate was cloned and expressed as recombinant protein. The recombinant DBLα-tag was used to activate PBMCs collected from each acute episode and from an annual cross-sectional survey performed after the acute malaria episode. In this article, we report that CD4+ T cell responses to the homologous DBLα-tag were induced in 75% of the children at the time of the acute episode and in 62% of the children at the following cross-sectional survey on average 235 d later. Furthermore, children who had induced DBLα-tag–specific CD4+IL-4+ T cells at the acute episode remained episode free for longer than children who induced other types of CD4+ T cell responses. These results suggest that a wide range of DBLα-tag–specific CD4+ T cell responses were induced in children with mild malaria and, in the case of CD4+IL-4+ T cell responses, were associated with protection from clinical episodes.
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CD4+ T cell responses to the Plasmodium falciparum erythrocyte membrane protein 1 in children with mild malaria
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Autor/in / Beteiligte Person: | Urban, Britta C. ; Gitau, Evelyn ; Requena, Pilar ; Kimani, Domtila ; Bull, Peter C. ; Eva N Kimani ; Tuju, James ; Olotu, Ally ; Stevenson, Liz ; Marsh, Kevin ; Karanja, Henry K. |
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Zeitschrift: | Journal of immunology (Baltimore, Md. : 1950), Jg. 192 (2014-02-01), Heft 4 |
Veröffentlichung: | 2014 |
Medientyp: | unknown |
ISSN: | 1550-6606 (print) ; 0022-1767 (print) |
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