Expression of Multidrug Resistant Gene (mdr-1/P-glycoprotein) in a Megakaryoblastic Cell Line, CMK, and its Enhancement During Megakaryocytic Differentiation
In: Leukemia & Lymphoma, Jg. 18 (1995), S. 515-520
Online
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Zugriff:
Multidrug resistance is a severe clinical problem in the chemotherapy of malignant disease. Acute megakaryoblastic leukemia (AMKL) is a rare form of childhood leukemia, and is often resistant to many anti-cancer chemotherapeutic drugs. Here we report the expression of the mdr-1/P-glycoprotein in a cell line, CMK, established from a patient with AMKL. Expression of mdr-1 mRNA in CMK11-5 cells, a well differentiated subline, was higher than in CMK6 cells, a poorly differentiated subline. The level of P-glycoprotein was also higher in CMK11-5 cells. The cytokines interferon-gamma (IFN-gamma), GM-CSF and IL-3, which were shown to induce megakaryocytic differentiation of CMK cells, enhanced the expression of the mdr-1 mRNA and levels of P-glycoprotein. These results imply that differentiated megakaryocytic cells may have higher levels of the P-glycoprotein expression, suggesting a possible normal physiological function of P-glycoprotein in mature megakaryocytes.
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Expression of Multidrug Resistant Gene (mdr-1/P-glycoprotein) in a Megakaryoblastic Cell Line, CMK, and its Enhancement During Megakaryocytic Differentiation
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Autor/in / Beteiligte Person: | Tsuruo, Takashi ; I Nobuyuki Miurai ; Kakuda, Harumi ; Sato, Takeyuki ; Niimi, Hiroo ; Ota, Setsuo ; Ito, Michihiro ; Fuse, Akira ; Asai, Takayoshi |
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Zeitschrift: | Leukemia & Lymphoma, Jg. 18 (1995), S. 515-520 |
Veröffentlichung: | Informa UK Limited, 1995 |
Medientyp: | unknown |
ISSN: | 1029-2403 (print) ; 1042-8194 (print) |
DOI: | 10.3109/10428199509059654 |
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