The Protective Effects of Sika Deer Antler Protein on Cisplatin-Induced Nephrotoxicity
In: Cellular Physiology and Biochemistry, Jg. 43 (2017), Heft 1, S. 395-404
Online
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Zugriff:
Background/Aims: This study measured the effect of Sika deer (Cervus nippon Temminck) antler protein (SDAPR), glycoproteins (SDAG), and polysaccharides (SDAPO) on cisplatin-induced cytotoxicity in HEK 293 cells, and investigated the effect of SDAPR against cisplatin-induced nephrotoxicity in mice. Methods: Cell viability was measured by MTT assay. ICR mice were randomly divided into five groups: control, cisplatin with vehicle, and cisplatin with SDAPR at three concentrations: 5, 10, or 20 mg/kg, p.o., 10 d. Cisplatin was injected on 7th day (25 mg/kg, i.p.). Renal function, oxidative stress, levels of inflammatory factors, and expression of apoptosis-related proteins were measured in vivo. Renal tissues were stained with TUNEL and H&E to observe renal cell apoptosis and pathological changes. Results: Pretreatment with SDAPR (125-2000 µg/mL) significantly improved cell viability, with an EC50 of approximately 1000 µg/mL. SDAPR also ameliorated cisplatin-induced histopatholo- gic changes, and decreased blood urea nitrogen (BUN) and creatinine (Cr) (P < 0.05). Western blotting analysis showed SDAPR clearly decreased expression levels of cleaved-caspase-3 and Bax, and increased the expression level of Bcl-2 (P < 0.01). Additionally, SDAPR markedly regulated oxidative stress markers and inflammatory cytokines (P
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The Protective Effects of Sika Deer Antler Protein on Cisplatin-Induced Nephrotoxicity
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Autor/in / Beteiligte Person: | Li, Wei ; Li, Wenqing ; Yang, Huihai ; Sun, Hang ; Wang, Lulu ; Zhang, Jing ; He, Xiaofeng |
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Zeitschrift: | Cellular Physiology and Biochemistry, Jg. 43 (2017), Heft 1, S. 395-404 |
Veröffentlichung: | Cell Physiol Biochem Press GmbH & Co KG, 2017 |
Medientyp: | unknown |
ISSN: | 1421-9778 (print) ; 1015-8987 (print) |
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