Characterization of the Duffy-Binding-Like Domain of Plasmodium falciparum Blood-Stage Antigen 332
In: Malaria Research and Treatment, Jg. 2011 (2011-09-19), S. 1-14
Online
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Zugriff:
Studies on Pf332, a major Plasmodium falciparum blood-stage antigen, have largely been hampered by the cross-reactive nature of antibodies generated against the molecule due to its high content of repeats, which are present in other malaria antigens. We previously reported the identification of a conserved domain in Pf332 with a high degree of similarity to the Duffy-binding-like (DBL) domains of the erythrocyte-binding-like (EBL) family. We here describe that antibodies towards Pf332-DBL are induced after repeated exposure to P. falciparum and that they are acquired early in life in areas of intense malaria transmission. Furthermore, a homology model of Pf332-DBL was found to be similar to the structure of the EBL-DBLs. Despite their similarities, antibodies towards Pf332-DBL did not display any cross-reactivity with EBL-proteins as demonstrated by immunofluorescence microscopy, Western blotting, and peptide microarray. Thus the DBL domain is an attractive region to use in further studies on the giant Pf332 molecule.
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Characterization of the Duffy-Binding-Like Domain of Plasmodium falciparum Blood-Stage Antigen 332
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Autor/in / Beteiligte Person: | Wahlgren, Mats ; Albrecht, Letusa ; Moll, Kirsten ; Kursula, Inari ; Angeletti, Davide ; Nilsson, Sandra ; Berzins, Klavs ; Chen, Qijun ; Sun, Xiaodong ; Jiang, Ning |
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Zeitschrift: | Malaria Research and Treatment, Jg. 2011 (2011-09-19), S. 1-14 |
Veröffentlichung: | Hindawi Limited, 2011 |
Medientyp: | unknown |
ISSN: | 2044-4362 (print) |
DOI: | 10.4061/2011/671439 |
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