Enzymatic modification of chitosan by cinnamic acids: Antibacterial activity against Ralstonia solanacearum
In: International journal of biological macromolecules, Jg. 87 (2015-12-16)
Online
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Zugriff:
This study aimed to identify chitosan polymers that have antibacterial activity against the bacterial wilt pathogen. The chitosan polymers were enzymatically synthesized using chitosan and five cinnamic acids (CADs): caffeic acid (CA), ferulic acid (FA), cinnamic acid (CIA), p-coumaric acid (COA) and chlorogenic acid (CHA), using laccase from Pleurotus ostreatus as a catalyst. The reaction was performed in a phosphate buffered solution under heterogenous reaction conditions. The chitosan derivatives (CTS-g-CADs) were characterized by FT-IR, XRD, TGA and SEM. FT-IR demonstrated that the reaction products bound covalently to the free amino groups or hydroxyl groups of chitosan via band of amide I or ester band. XRD showed a reduced packing density for grafted chitosan comparing to original chitosan. TGA demonstrated that CTS-g-CADs have a higher thermostability than chitosan. Additionally, chitosan and its derivatives showed similar antibacterial activity. However, the IC50 value of the chitosan-caffeic acid derivative (CTS-g-CA) against the mulberry bacterial wilt pathogen RS-5 was 0.23mg/mL, which was two-fifths of the IC50 value of chitosan. Therefore, the enzymatically synthesized chitosan polymers can be used to control plant diseases in biotechnological domains.
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Enzymatic modification of chitosan by cinnamic acids: Antibacterial activity against Ralstonia solanacearum
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Autor/in / Beteiligte Person: | Zhou, Yu ; Zheng, Yu ; Li, Changlong ; Wu, Fu-An ; Yang, Caifeng ; Wang, Jun ; Sheng, Sheng |
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Zeitschrift: | International journal of biological macromolecules, Jg. 87 (2015-12-16) |
Veröffentlichung: | 2015 |
Medientyp: | unknown |
ISSN: | 1879-0003 (print) |
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