Synchronous activation of the alleles coding for the S-form of the NADP-dependent malate dehydrogenase during mouse embryogenesis
In: Human Genetics, Jg. 12 (1971), S. 162-166
Online
unknown
Zugriff:
The first appearance during mouse development of different allelic products was studied using the supernatant (S-)form of NADP-dependent MDH. Heterozygote offspring were produced by cross-breeding the strains C3H and CBA, and the embryos were taken at various developmental stages. The electrophoretic examination revealed no enzyme activity in unfertilized ova and preimplantation stages. After implantation, an isoenzyme pattern was seen for the first time at day 10 (228 hrs after fertilization), representing the 3 different heteromers of this tetrameric enzyme in binomial distribution. 240 hrs after fertilization, the 5-banded pattern to be expected in the heterozygotes was complete. Measurements of enzyme activity revealed a strong activity increase at about 240 hrs. The results indicate that the S-form of NADP-MDH appears rather late in development and that the two alleles tested at this gene locus commence functioning synchronously.
| Titel: |
Synchronous activation of the alleles coding for the S-form of the NADP-dependent malate dehydrogenase during mouse embryogenesis
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| Autor/in / Beteiligte Person: | Wolf, Ulrich ; Engel, Wolfgang |
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| Zeitschrift: | Human Genetics, Jg. 12 (1971), S. 162-166 |
| Veröffentlichung: | Springer Science and Business Media LLC, 1971 |
| Medientyp: | unknown |
| ISSN: | 1432-1203 (print) ; 0340-6717 (print) |
| DOI: | 10.1007/bf00291472 |
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