The impact of spike N501Y mutation on neutralizing activity and RBD binding of SARS-CoV-2 convalescent serum
In: EBioMedicine, Jg. 71 (2021-09-01)
Online
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Zugriff:
BACKGROUND: Several SARS-CoV-2 lineages with spike receptor binding domain (RBD) N501Y mutation have spread globally. We evaluated the impact of N501Y on neutralizing activity of COVID-19 convalescent sera and on anti-RBD IgG assays. METHODS: The susceptibility to neutralization by COVID-19 patients' convalescent sera from Hong Kong were compared between two SARS-CoV-2 isolates (B117-1/B117-2) from the α variant with N501Y and 4 non-N501Y isolates. The effect of N501Y on antibody binding was assessed. The performance of commercially-available IgG assays was determined for patients infected with N501Y variants. FINDINGS: The microneutralization antibody (MN) titers of convalescent sera from 9 recovered COVID-19 patients against B117-1 (geometric mean titer[GMT],80; 95% CI, 47-136) were similar to those against the non-N501Y viruses. However, MN titer of these serum against B117-2 (GMT, 20; 95% CI, 11-36) was statistically significantly reduced when compared with non-N501Y viruses (P
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The impact of spike N501Y mutation on neutralizing activity and RBD binding of SARS-CoV-2 convalescent serum
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| Autor/in / Beteiligte Person: | To, Kelvin K. W. ; Bobo Wing-Yee Mok ; Hung, Ivan ; Tsoi, Hoi-Wah ; To, Wing-Kin ; Vincent C.C. Cheng ; David Christopher Lung ; Jonathan Daniel Ip ; Yau, Yat-Sun ; Anthony Raymond Tam ; Lu, Lu ; Chan, Wan-Mui ; Chen, Lin-Lei ; Ricky Ruiqi Zhang ; Ip, Tak-Chuen ; Gilman Kit Hang Siu ; Chan, Kwok-Hung ; Larry Lap-Yip Lee ; Cai, Jian-Piao ; Rosana W.S. Poon ; Mike Yat-Wah Kwan ; Owen Tak-Yin Tsang ; Yi, Haisu ; Yuen, Kwok-Yung ; Allen Wing-Ho Chu |
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| Zeitschrift: | EBioMedicine, Jg. 71 (2021-09-01) |
| Veröffentlichung: | Elsevier, 2021 |
| Medientyp: | unknown |
| ISSN: | 2352-3964 (print) |
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