A comparative fluorescent beacon-based method for serum microRNA quantification
In: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, Jg. 31 (2015-03-14), Heft 3
Online
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Zugriff:
Circulating serum microRNAs (miRNAs) are promising biomarkers for disease diagnosis. The quantification of the serum miRNA copy number is a challenge due to the presence of low levels in the serum. Here, we report on a direct measurement of the miRNA copy number from human serum using a locked nucleic acid (LNA) modified beacon probe with a single step using fluorescence spectroscopy and microscopy. We had used a minimum volume of 0.1 μL healthy human serum and retinoblastoma serum to show the biological variation of the miRNA copy number.
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A comparative fluorescent beacon-based method for serum microRNA quantification
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Autor/in / Beteiligte Person: | Salim, Bindu ; Elchuri, Sailaja ; Krishnakumar, Subramanian ; Narayanan, Janakiraman ; Beta, Madhu |
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Zeitschrift: | Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, Jg. 31 (2015-03-14), Heft 3 |
Veröffentlichung: | 2015 |
Medientyp: | unknown |
ISSN: | 1348-2246 (print) |
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