A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs
In: Life Sciences, Jg. 67 (2000-10-01), S. 2889-2896
Online
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Zugriff:
The aqueous and methanol extracts of thirty-one herbs traditionally used as anti-fever remedies in China were screened for their in vitro inhibition on human immunodeficiency virus type-1 protease (HIV-1 PR). The activity of recombinant HIV-1 protease was determined by sequence-specific cleavage at the Tyr-Pro bond of the fluorogenic substrate (Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)- Arg) or by HPLC anaylsis of the cleavage products after incubation of the enzyme with a synthetic peptide substrate (Acetyl-Ser-Gln-Asn-Tyr-Pro-Val-Val-amide). Among the herbal extracts examined, the aqueous extracts of Prunella vulgaris and Scutellaria baicalensis and the methanol extracts of Woodwardia unigemmata, Paeonica suffruticosa and Spatholobus suberectus elicited significant inhibition (>90%) at a concentration of 200 microg/ml.
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A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs
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Autor/in / Beteiligte Person: | Ip, D.T.M. ; Lam, T.L. ; David Chi Cheong Wan ; Lam, M.L. ; Tzi Bun Ng ; Fong, Wing-Ping ; Au, T.K. |
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Zeitschrift: | Life Sciences, Jg. 67 (2000-10-01), S. 2889-2896 |
Veröffentlichung: | Elsevier BV, 2000 |
Medientyp: | unknown |
ISSN: | 0024-3205 (print) |
DOI: | 10.1016/s0024-3205(00)00864-x |
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