Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica
In: Biotechnology for Biofuels, Jg. 8 (2015-11-25)
Online
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Zugriff:
Background Production of valuable metabolites by Yarrowia lipolytica using renewable raw materials is of major interest for sustainable food and energy. Galactose is a monosaccharide found in galactomannans, hemicelluloses, gums, and pectins. Results Yarrowia lipolytica was found to express all the Leloir pathway genes for galactose utilization, which encode fully functional proteins. Gene organization and regulation in Y. lipolytica resembles filamentous fungi rather than Saccharomyces cerevisiae. After Y. lipolytica was grown on mixture of glucose and galactose, it was then able to metabolize galactose, including when glucose concentrations were higher than 4 g/L. However, glucose was still the preferred carbon source. Nonetheless, a strain overexpressing the four ylGAL genes of the Leloir pathway was able to efficiently use galactose as its sole carbon source. This mutant was used to produce citric acid and lipids from galactose; the yields were comparable to or greater than that obtained for the parental strain (W29) on glucose. Conclusions The construction of a Y. lipolytica strain able to produce citric acid and lipids from galactose is a very important step in bypassing issues related to the use of food-based substrates in industrial applications. Electronic supplementary material The online version of this article (doi:10.1186/s13068-015-0370-4) contains supplementary material, which is available to authorized users.
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Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica
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Autor/in / Beteiligte Person: | Nicaud, Jean-Marc ; Lazar, Zbigniew ; Gamboa-Meléndez, Heber ; Anne-Marie Crutz-Le Coq ; Neuvéglise, Cécile ; MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech ; Department of Biotechnology and Food Microbiology ; Wroclaw University of Environmental and Life Sciences ; French NationalResearch Agency (Agence Nationale de la Recherche ; Investissements d'avenir program) ANR-11-BTBR-0003 ; French National Institute for Agricultural Research (INRA) ; European Project: 267196,EC:FP7:PEOPLE,FP7-PEOPLE-2010-COFUND,AGREENSKILLS(2012) |
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Zeitschrift: | Biotechnology for Biofuels, Jg. 8 (2015-11-25) |
Veröffentlichung: | Springer Science and Business Media LLC, 2015 |
Medientyp: | unknown |
ISSN: | 1754-6834 (print) |
DOI: | 10.1186/s13068-015-0370-4 |
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