Cloning in a plasmid of an MMTV from a wild Chinese mouse: sequencing of the viral LTR
In: Virus research, Jg. 33 (1994-08-01), Heft 2
Online
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Zugriff:
Plasmid subcloning by conventional techniques of full length exogenous mouse mammary tumor viruses (MMTV) has not been realized because of the involvement of host-mediated structural changes in the viral gag gene. To circumvent this problem, an alternative subcloning method, excision of phagemid (pBluescript SK) from lambda ZAP II, was successfully used to subclone a novel exogenous MMTV (JYG-MMTV) provirus fragment containing an intact gag gene. Sequence analysis revealed that the LTR of this virus is significantly different from the LTR of C3H-MMTV in the U3 region.
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Cloning in a plasmid of an MMTV from a wild Chinese mouse: sequencing of the viral LTR
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Autor/in / Beteiligte Person: | Sarkar, Nurul H. ; Imai, Shunsuke ; Xu, Lai ; Haga, Satomi |
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Zeitschrift: | Virus research, Jg. 33 (1994-08-01), Heft 2 |
Veröffentlichung: | 1994 |
Medientyp: | unknown |
ISSN: | 0168-1702 (print) |
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