Enhanced Recovery of Listeria from Dairy-Plant Processing Environments through Combined Use of Repair Enrichment and Selective Enrichment/Detection Procedures
In: Journal of food protection, Jg. 58 (2019-05-30), Heft 4
Online
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Zugriff:
The efficacy of using a repair step to increase sensitivity of recovery of injured Listeria from environmental sources in dairy processing plants was investigated. The USDA-FSIS Listeria isolation protocol using UVM-modified Listeria enrichment broth medium University of Vermont (UVM) for primary enrichment was the standard method chosen for comparison. UVM broth was used in conjunction with rapid methods (Organon Teknika and Gene-Trak™), following manufacturer's guidelines. Listeria Repair Broth (LRB) was used as the repair enrichment medium in modified protocols of the standard and rapid procedures. LRB employs a nonselective period (2–5 hours) for repair of injured Listeria prior to selective-agent addition. Of 80 environmental sites positive by any method, UVM and LRB showed similar recovery rates (87.5% and 88.8%, respectively). Thus LRB provided little advantage over current procedures for use in contaminated sites. UVM was superior when used in conjunction with either rapid method. The USDA and mod...
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Enhanced Recovery of Listeria from Dairy-Plant Processing Environments through Combined Use of Repair Enrichment and Selective Enrichment/Detection Procedures
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Autor/in / Beteiligte Person: | Flanders, Kathy J. ; Pritchard, Todd J. ; Donnelly, Catherine W. |
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Zeitschrift: | Journal of food protection, Jg. 58 (2019-05-30), Heft 4 |
Veröffentlichung: | 2019 |
Medientyp: | unknown |
ISSN: | 1944-9097 (print) |
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