Validated method for determination of eight banned nitroimidazole residues in natural casings by LC/MS/MS with solid-phase extraction
In: Journal of AOAC International, Jg. 92 (2009-06-03), Heft 2
Online
unknown
Zugriff:
A sensitive method based on solid-phase extraction-liquid chromatography-tandem mass spectrometry interfaced with electrospray ionization (SPE-LC-MS/MS-ESI) was developed for the simultaneous determination of 8 banned nitroimidazole (NOZ) drugs including metronidazole (MNZ), ronidazole (RNZ), dimetridazole (DMZ), tinidazole, ornidazole, secnidazole, metronidazole-OH (MNZOH, the metabolite of MNZ), and 2-hydroxymethyl-1-methyl-5-nitroimidazole (HMMNI, the metabolite of RNZ and DMZ) in natural casings. After extraction with ethyl acetate and evaporation, the NOZs were reconstituted in ethyl acetate and purified on a strong cation-exchange SPE column, and then LC/MS/MS analysis was performed by positive ESI applying multiple reaction monitoring of 2 transition reactions for each compound. The method was validated according to the European Union requirements (Commission Decision 2002/657/EC). Specificity, linearity, decision limit (CC), detection capability (CC), accuracy, and precision were determined. Average recoveries of the 8 NOZs from natural animal casing fortified at 3 levels (0.1, 0.5, and 1 g/kg) ranged from 87.3 to 116.5. The calculated CC for NOZs ranged from 0.029 to 0.049 g/kg, and CC ranged from 0.049 to 0.083 g/kg. Repeatability was in the range of 3.3510.1, and within-laboratory reproducibility was
Titel: |
Validated method for determination of eight banned nitroimidazole residues in natural casings by LC/MS/MS with solid-phase extraction
|
---|---|
Autor/in / Beteiligte Person: | Guo, Chunhai ; Sun, Hanwen ; Chen, Ruichun ; Wang, Fengchi ; Ai, Lianfeng |
Link: | |
Zeitschrift: | Journal of AOAC International, Jg. 92 (2009-06-03), Heft 2 |
Veröffentlichung: | 2009 |
Medientyp: | unknown |
ISSN: | 1060-3271 (print) |
Schlagwort: |
|
Sonstiges: |
|