Stem-cell factor regulates the expression of cyclin A and retinoblastoma gene product in the growth and differentiation pathway of human megakaryocytic cells
In: Journal of Cancer Research and Clinical Oncology, Jg. 122 (1996-08-01), S. 445-452
Online
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Zugriff:
The biological effects of c-kit ligand (stem-cell factor: SCF) on an immortalized human megakaryocytic cell line (CMK) was evaluated using methods including the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, surface marker analysis, DNA cell-cycle analysis and immunoblotting. SCF stimulated the growth of CMK cells. Incubation with SCF resulted in increased expression of IIb/IIIa platelet-related glycoprotein (gpIIb, IIIa), indicating enhanced differentiation of CMK cells. Treatment of CMK cells with SCF resulted in a decrease in the subpopulation in the G1 phase, with a reciprocal increase in those in the S phase and the G2 + M phase. Moreover, SCF significantly increased cellular expression of cyclin A, a regulatory subunit of cyclin-dependent protein kinase (CDK), and the ratio of phosphorylated/dephosphorylated retinoblastoma gene product (RB protein). These results suggest that SCF stimulates the growth and differentiation of megakaryocytic cells possibly through mechanisms related to the activation of cell-cycle-dependent serine/threonine kinase and inactivation of the nuclear tumor-suppressor gene product.
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Stem-cell factor regulates the expression of cyclin A and retinoblastoma gene product in the growth and differentiation pathway of human megakaryocytic cells
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Autor/in / Beteiligte Person: | Yamamoto, Yoshihiko ; Sone, Saburo ; Yoshida, Seiji ; Takahashi, Yasutake ; Takaue, Yoichi ; Ogura, Takeshi ; Shinohara, Akinori ; Yamamoto, Akiyoshi ; Shimizu, Eiji |
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Zeitschrift: | Journal of Cancer Research and Clinical Oncology, Jg. 122 (1996-08-01), S. 445-452 |
Veröffentlichung: | Springer Science and Business Media LLC, 1996 |
Medientyp: | unknown |
ISSN: | 1432-1335 (print) ; 0171-5216 (print) |
DOI: | 10.1007/bf01187155 |
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