Selective isolation of multiply charged peptides: a confident strategy for protein identification using a linear trap quadrupole mass spectrometer
In: European journal of mass spectrometry (Chichester, England), Jg. 18 (2013-05-10), Heft 6
Online
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Zugriff:
In this work, a method devised for the selective isolation of multiply-charged peptide applied to a complex protein mixture was evaluated for the first time using a mass spectrometer with low resolution (LTQ). In this procedure, all primary amino groups of tryptic peptides derived from human liver tissue interstitial fluid (TIF) are blocked, restricting their positive charge, at acidic pH, to the presence of histidine and arginine residues. After strong cation exchange chromatography, multiply-charged peptides (#R + #H > 1) are retained in the column and separated with high selectivity from singly (#R + #H = 1) and neutral peptides (#R + #H = 0) which are collected together in the flow-through. Using liquid chromatography electrospray ionization tandem mass spectrometry analysis the retained fraction displayed a 95% enrichment of multiply charged peptides while in the flow-through; only 4% of multiply-charged peptides were identified.
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Selective isolation of multiply charged peptides: a confident strategy for protein identification using a linear trap quadrupole mass spectrometer
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Autor/in / Beteiligte Person: | He, Fuchu ; Sanchez, Aniel ; Perez-Riverol, Yasset ; Sun, Wei ; Besada, Vladimir ; Jorge Fernandez de-Cossio ; Luis Javier González ; Padrón, Gabriel ; Ma, Jie ; Jiang, Ying ; Betancourt, Lázaro |
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Zeitschrift: | European journal of mass spectrometry (Chichester, England), Jg. 18 (2013-05-10), Heft 6 |
Veröffentlichung: | 2013 |
Medientyp: | unknown |
ISSN: | 1469-0667 (print) |
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