Long noncoding RNA lnc-NAP sponges mmu-miR-139-5p to modulate Nanog functions in mouse ESCs and embryos
In: RNA Biology, Jg. 18 (2020-10-23), S. 875-887
Online
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Zugriff:
The pluripotency of embryonic stem cells (ESCs) is controlled by a multilayer regulatory network, of which the key factors include core pluripotency genes Oct4, Sox2 and Nanog, and multiple microRNAs (miRNAs). Recently, long noncoding RNAs (lncRNAs) have been discovered as a class of new regulators for ESCs, and some lncRNAs could function as competing endogenous RNAs (ceRNAs) to regulate mRNAs by competitively binding to miRNAs. Here, we identify mmu-miR-139-5p as a new regulator for Nanog by targeting Nanog 3′ untranslated region (UTR) to repress Nanog expression in mouse ESCs and embryos. Such regulation could be released by an ESC-specifically expressed ceRNA named lnc-NAP. The expression of lnc-NAP is activated by OCT4, SOX2, as well as NANOG through promoter binding. Downregulation of lnc-NAP reduces Nanog abundance, which leads to decreased pluripotency of mouse ESCs and embryonic lethality. These results reveal lnc-NAP as a new regulator for Nanog in mouse ESCs, and uncover a feed-forward regulatory loop of Nanog through the participation of lnc-NAP.
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Long noncoding RNA lnc-NAP sponges mmu-miR-139-5p to modulate Nanog functions in mouse ESCs and embryos
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Autor/in / Beteiligte Person: | Li, Ang ; Zhang, Hao ; Xia, Baolong ; Wang, Leyun ; Tong, Man ; Luo, Guan-Zheng ; Wang, Meng ; Feng, Gui-Hai ; Yang, Yun-Gui ; Zhang, Zeyu ; Wang, Xiu-Jie ; Wan, Haifeng ; Xie, Dongfang ; Zhou, Qi |
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Zeitschrift: | RNA Biology, Jg. 18 (2020-10-23), S. 875-887 |
Veröffentlichung: | Informa UK Limited, 2020 |
Medientyp: | unknown |
ISSN: | 1555-8584 (print) ; 1547-6286 (print) |
DOI: | 10.1080/15476286.2020.1827591 |
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