The Drosophila melanogaster suppressor of Hairy-wing protein binds to specific sequences of the gypsy retrotransposon
In: Genes & Development, Jg. 2 (1988-11-01), S. 1414-1423
Online
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Zugriff:
Mutations at the suppressor of Hairy-wing [su(Hw), 3-54.8] locus reverse the phenotype of second-site mutations induced by the gypsy transposable element in Drosophila melanogaster. This gene encodes a protein with a predicted molecular weight of 109,000 that contains an acidic domain and 12 copies of the DNA-binding 'Zn finger' motif. The su(Hw) protein was overexpressed in Escherichia coli and Drosophila cells, and partially purified. It was shown to interact specifically in vitro with a 367-bp DNA fragment that contains 12 copies of the sequence PyPuTTGCATACCPy located in the 5'-untranslated region of gypsy, between the 5' long terminal repeat (LTR) and the first ATG initiation codon. This sequence shows striking homology to some mammalian transcriptional enhancer elements, supporting a role for the su(Hw) protein in the control of gypsy transcription. In addition, the su(Hw) protein is present at approximately 100-200 sites on Drosophila polytene chromosomes, suggesting that it also interacts in vivo with DNA and might be involved functionally in the regulation of normal cellular genes.
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The Drosophila melanogaster suppressor of Hairy-wing protein binds to specific sequences of the gypsy retrotransposon
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Autor/in / Beteiligte Person: | Spana, C ; Harrison, Douglas A. ; Corces, Victor G. |
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Zeitschrift: | Genes & Development, Jg. 2 (1988-11-01), S. 1414-1423 |
Veröffentlichung: | Cold Spring Harbor Laboratory, 1988 |
Medientyp: | unknown |
ISSN: | 1549-5477 (print) ; 0890-9369 (print) |
DOI: | 10.1101/gad.2.11.1414 |
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