Immobilization of surface non-affinitive protein onto a metal surface by an external electric field
In: Journal of bioscience and bioengineering, Jg. 129 (2019-05-09), Heft 3
Online
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Zugriff:
We investigated an alternate technique to coat the surface with a protein having no surface affinity, without the use of any exotic chemical agents. An external electric field was utilized to prepare the protein coating on a metal substrate. Stainless steel (St) substrate and lysozyme (LSZ) were used as the surface to be coated and the model non-adsorptive protein, respectively. Dynamics of the adsorption of LSZ on the St surface in the presence and absence of an external electric potential (EEP) were monitored by in-situ ellipsometry. Applying negative surface potential (−0.4 V vs Ag/AgCl) forced the adsorption of LSZ onto the St surface where LSZ did not adsorb without applying any EEP. The repetition of the EEP-application and -cut-off indicated the controllability of the LSZ coating amount depending on the total duration of the EEP-application. The coated LSZ largely remained bound to the surface even by the cut-off of the external electric field, the ratio of which to the detached amount was roughly constant (approximately 7:3). Furthermore, the LSZ coated surface on the St substrate was found to be reversibly switched between being affinitive and non-affinitive to a typical model protein adsorbate (bovine serum albumin) by the EEP-application and cut-off.
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Immobilization of surface non-affinitive protein onto a metal surface by an external electric field
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Autor/in / Beteiligte Person: | Nakao, Hajime ; Imanaka, Hiroyuki ; Imamura, Koreyoshi ; Tramis, Olivier ; Ishida, Naoyuki ; Iizuka, Ryosuke |
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Zeitschrift: | Journal of bioscience and bioengineering, Jg. 129 (2019-05-09), Heft 3 |
Veröffentlichung: | 2019 |
Medientyp: | unknown |
ISSN: | 1347-4421 (print) |
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