Detection of IL-6 and its receptor mRNAs in a megakaryocytic cell line, CMK, by an RT-nested PCR
In: Annals of hematology, Jg. 69 (1994-11-01), Heft 5
Online
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Zugriff:
An autocrine loop in a megakaryocytic cell line, CMK, has been proposed by Fuse et al. They detected IL-6 mRNA by means of Northern blotting, but not IL-6 receptor (IL-6R) mRNA. I also failed to detect IL-6R mRNA by the same means. In this study I showed the expression of IL-6 and its receptor mRNAs by means of reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) with an automated electrophoresis PhastSystem. These two procedures considerably enhanced detection sensitivity. The proliferation and the differentiation of CMK cells are augmented by IL-6 and inhibited by anti-IL-6 antibody. This study continued the autocrine theory of Fuse et al. in CMK cells. Moreover, this is the first detection of IL-6R mRNA in CMK.
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Detection of IL-6 and its receptor mRNAs in a megakaryocytic cell line, CMK, by an RT-nested PCR
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Autor/in / Beteiligte Person: | Nakayama, K |
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Zeitschrift: | Annals of hematology, Jg. 69 (1994-11-01), Heft 5 |
Veröffentlichung: | 1994 |
Medientyp: | unknown |
ISSN: | 0939-5555 (print) |
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