Triamcinolone acetonide protects auditory hair cells from 4-hydroxy-2,3-nonenal (HNE) ototoxicity in vitro
In: Acta oto-laryngologica, Jg. 126 (2006-06-29), Heft 7
Online
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Zugriff:
Triamcinolone acetonide crystalline suspension (e.g. Volon A) was not ototoxic to the auditory hair cells present within organ of Corti explants and protected them from an ototoxic molecule, i.e. 4-hydroxy-2,3-nonenal (HNE), that is produced within the organ of Corti as a result of oxidative stress-induced damage.To test the corticosteroid, triamcinolone acetonide, for ototoxicity and otoprotective capacity in organ of Corti explants.Organ of Corti explants excised from 4-day-old rats were the test system, HNE was the ototoxin challenge. Hair cell integrity counts were performed with fluorescent microscopy on fixed explants stained with FITC-labeled phalloidin. Statistical significance was set at p0.05.Triamcinolone acetonide did not affect hair cell integrity in the organ of Corti explants and it provided a high level of protection of hair cells against the ototoxic effects of a damaging level of HNE as determined by hair cell density counts.
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Triamcinolone acetonide protects auditory hair cells from 4-hydroxy-2,3-nonenal (HNE) ototoxicity in vitro
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Autor/in / Beteiligte Person: | Ruiz, Jose ; Guzman, Jose F. ; Polak, Marek ; Garnham, Carolyn ; Thomas R. Van De Water ; Balkany, Thomas J. ; Eshraghi, Adrien A. |
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Zeitschrift: | Acta oto-laryngologica, Jg. 126 (2006-06-29), Heft 7 |
Veröffentlichung: | 2006 |
Medientyp: | unknown |
ISSN: | 0001-6489 (print) |
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