Advanced Fluorescent Polymer Probes for the Site-Specific Labeling of Proteins in Live Cells Using the HaloTag Technology
In: ACS Omega, Jg. 4 (2019), Heft 7, S. 12841-12847
Online
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Zugriff:
International audience; We report the site-specific and covalent bioconjugation of fluorescent polymer chains to proteins in live cells using the HaloTag technology. Polymer chains bearing a Halo-ligand precisely located at their α-chain-end were synthesized in a controlled manner owing to the RAFT polymerization process. They were labeled in lateral position by several organic fluorophores such as AlexaFluor 647. The resulting Haloligand polymer probe was finally shown to selectively recognize and label HaloTag proteins present at the membrane of live cells using confocal fluorescence microscopy. Such a polymer bioconjugation approach holds great promises for various applications ranging from cell imaging to cell surface functionalization.
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Advanced Fluorescent Polymer Probes for the Site-Specific Labeling of Proteins in Live Cells Using the HaloTag Technology
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| Autor/in / Beteiligte Person: | Raimondi, Andrea ; Fabre, Laura ; Marie Therese Charreyre ; Berki, Thomas ; Bakunts, Anush ; Ladavière, Catherine ; Orsi, Andrea ; Favier, Arnaud ; Eelco van Anken ; Duret, Damien ; Ingénierie des Matériaux Polymères (IMP) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon) ; Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) ; Systèmes Macromoléculaires et Physiopathologie Humaine (SMPH) ; BIOMERIEUX-Centre National de la Recherche Scientifique (CNRS) ; San Raffaele Scientific Institute ; Vita-Salute San Raffaele University and Center for Translational Genomics and Bioinformatics ; Ingénierie des Matériaux Polymères - Laboratoire des Matériaux Macromoléculaires (IMP-LMM) ; Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS) ; Berki, T ; Bakunts, A ; Duret, D ; Fabre, L ; Ladaviere, C ; Orsi, A ; Charreyre, Mt ; Raimondi, A ; van Anken, E ; Favier, A |
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| Zeitschrift: | ACS Omega, Jg. 4 (2019), Heft 7, S. 12841-12847 |
| Veröffentlichung: | HAL CCSD, 2019 |
| Medientyp: | unknown |
| ISSN: | 2470-1343 (print) |
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