Detection and Structural Characterization of SFAHFA Homologous Series in Mouse Colon Contents by LTQ-Orbitrap-MS and Their Implication in Influenza Virus Infection
In: Journal of the American Society for Mass Spectrometry, Jg. 32 (2021-06-25), S. 2196-2205
Online
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Zugriff:
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with promising physiological functions in mammals. We previously introduced a new type of lipids to this family called short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs), branching specific to the C2 carbon of a long-chain fatty acid (≥C20). In this study, we discovered a homologous series of SFAHFAs comprising C16-C26 hydroxy fatty acids esterified with short-chain fatty acids (C2-C5) in mouse colon contents. The detected SFAHFAs were characterized by high-resolution mass spectrometry with MSn analysis. The double-bond position of monounsaturated SFAHFAs was determined by the epoxidation reaction of samples with m-chloroperoxybenzoic acid and their MSn analysis. Further, the measurement of SFAHFA concentration in the colon contents of mice infected with influenza A/Puerto Rico/8/34 (H1N1; PR8) virus revealed a significant increase in their levels compared to native control. A strong correlation was observed between hydroxy fatty acid and SFAHFAs. Detection, characterization, and profiling of these new SFAHFA levels in relation with pandemic H1N1; PR8 influenza virus will contribute to the in-depth study of their function and metabolism.
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Detection and Structural Characterization of SFAHFA Homologous Series in Mouse Colon Contents by LTQ-Orbitrap-MS and Their Implication in Influenza Virus Infection
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Autor/in / Beteiligte Person: | Ohno, Marumi ; Liang, Chongsheng ; Chiba, Hitoshi ; Hui, Shu-Ping ; Gowda, Divyavani ; Siddabasave Gowda B. Gowda |
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Zeitschrift: | Journal of the American Society for Mass Spectrometry, Jg. 32 (2021-06-25), S. 2196-2205 |
Veröffentlichung: | American Chemical Society (ACS), 2021 |
Medientyp: | unknown |
ISSN: | 1879-1123 (print) ; 1044-0305 (print) |
DOI: | 10.1021/jasms.1c00138 |
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