Transcriptomics supports local sensory regulation in the antenna of the kissing-bug Rhodnius prolixus
In: CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET BMC Genomics BMC Genomics, BioMed Central, 2020, 21 (1), pp.101. ⟨10.1186/s12864-020-6514-3⟩ bioRxiv SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP, Jg. 21 (2020), Heft 1, S. 1-14
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Zugriff:
Background: Rhodnius prolixus has become a model for revealing the molecular bases of insect sensory biology due to the publication of its genome and its well-characterized behavioural repertoire. Gene expression modulation underlies behaviour-triggering processes at peripheral and central levels. Still, the regulation of sensory-related gene transcription in sensory organs is poorly understood. Here we study the genetic bases of plasticity in antennal sensory function, using R. prolixus as an insect model. Results: Antennal expression of neuromodulatory genes such as those coding for neuropeptides, neurohormones and their receptors was characterized in fifth instar larvae and female and male adults by means of RNA-Sequencing (RNASeq). New nuclear receptor and takeout gene sequences were identified for this species, as well as those of enzymes involved in the biosynthesis and processing of neuropeptides and biogenic amines. Conclusions: We report a broad repertoire of neuromodulatory and neuroendocrine-related genes expressed in the antennae of R. prolixus and suggest that they may serve as the local basis for modulation of sensory neuron physiology. Diverse neuropeptide precursor genes showed consistent expression in the antennae of all stages studied. Future studies should characterize the role of these modulatory components acting over antennal sensory processes to assess the relative contribution of peripheral and central regulatory systems on the plastic expression of insect behaviour.
Centro Regional de Estudios Genómicos
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Transcriptomics supports local sensory regulation in the antenna of the kissing-bug Rhodnius prolixus
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| Autor/in / Beteiligte Person: | Sterkel, Marcos ; Jose Manuel Latorre-Estivalis ; Lorenzo, Marcelo G. ; Ons, Sheila ; Fundação Oswaldo Cruz (FIOCRUZ) ; Réseau International des Instituts Pasteur (RIIP) ; Centro Regional de Estudios Genómicos [universidad Nacional de La Plata, Argentina] (CREG-UNLP) ; Universidad Nacional de la Plata [Argentine] (UNLP) ; LE STUDIUM (LE STUDIUM) ; Bureau de Recherches Géologiques et Minières (BRGM) (BRGM)-Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de recherche pour le développement [IRD] : UR-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) ; This project has been supported by LE STUDIUM Loire Valley Institute for Advanced Studies, Orléans & Tours, France with funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 665790Authors are indebted to INCTEM (Project number: 573959/2008–0), FAPEMIG (Project number: APQ-01359-11), PROEP-FIOCRUZ (Project number: 401973/2012–3), CNPq (Project number: 483805/2013–0), Le Studium for a granting research fellowship to MGL (Short Term Contract of Employment N° 2017–2001-179 - Y17F16), FIOCRUZ Visiting researcher fellowship program (fellowship to JML-E 550017/2015–17), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) postdoctoral fellowship (fellowship to JML-E 2015–2017) and Agencia Nacional de Promoción Científica y Tecnológica (Project number: PICT 2016–3103). JML-E., MS and SO are researchers from CONICET. All these funds provided financial support in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. ; The authors wish to thank the Program for Technological Development in Tools for Health-PDTIS-FIOCRUZ for having facilitated the use of its facilities. We wish to thank Ivana Helena Rocha Oliveira for her help in processing samples used in our experiments, Prof. Claudio Lazzari for kindly providing comments on an earlier version of the manuscript, and Dr. Gabriel da Rocha Fernandes and Fausto Gonçalves dos Santos for their help and support in the bioinformatic analyses ; Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de recherche pour le développement [IRD] : UR-Centre National de la Recherche Scientifique (CNRS) |
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| Zeitschrift: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET BMC Genomics BMC Genomics, BioMed Central, 2020, 21 (1), pp.101. ⟨10.1186/s12864-020-6514-3⟩ bioRxiv SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP, Jg. 21 (2020), Heft 1, S. 1-14 |
| Veröffentlichung: | Springer Science and Business Media LLC, 2020 |
| Medientyp: | unknown |
| ISSN: | 1471-2164 (print) |
| DOI: | 10.1186/s12864-020-6514-3 |
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