Proteomic analysis of glutamine-treated human intestinal epithelial HCT-8 cells under basal and inflammatory conditions
In: PROTEOMICS, Jg. 6 (2006-05-31), S. 3926-3937
Online
unknown
Zugriff:
International audience; Glutamine (Gln) promotes intestinal growth and maintains gut structure and function, especially in situations of injury and during inflammation. Several mechanisms could contribute to Gln protective effects on gut. Proteomics enable us to characterize differentially expressed proteins in tissues in response to modifications of the biological or nutritional environment. Gln effects on the human intestinal epithelial HCT-8 cell line proteome were assessed under basal and proinflammatory conditions. The 2-DE gels were obtained and compared. Proteins were identified by MS and using databases. About 1200 spots were detected in both 2- and 10-mM Gln concentrations. Under basal conditions, 24 proteins were differentially expressed in response to Gln. Half of these proteins were implicated in protein biosynthesis or proteolysis and 20% in membrane trafficking. Under proinflammatory conditions, 27 proteins were up- or down-regulated by Gln 10 mM. From these proteins, 40% were involved in protein biosynthesis or proteolysis, 16% in membrane trafficking, 8% in cell cycle and apoptosis mechanisms and 8% in nucleic acid metabolism. This study provides the first holistic picture of proteome modulation by Gln in a human enterocytic cell line under basal and proinflammatory conditions, and supports further evaluation of nutritional modulation of intestinal proteome in humans.
Titel: |
Proteomic analysis of glutamine-treated human intestinal epithelial HCT-8 cells under basal and inflammatory conditions
|
---|---|
Autor/in / Beteiligte Person: | Charlionet, Roland ; Marion, Rachel ; Vaudry, Hubert ; Deniel, Nicolas ; Cosquer, David ; Leprince, Jérôme ; Thébault, Sandrine ; Tron, François ; Déchelotte, Pierre ; Ducrotté, Philippe ; Appareil Digestif Environnement Nutrition (ADEN ) ; Université de Rouen Normandie (UNIROUEN) ; Normandie Université (NU)-Normandie Université (NU) ; Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (IFRMP 23) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Lutte Contre le Cancer Henri Becquerel Normandie Rouen (CLCC Henri Becquerel)-Université de Rouen Normandie (UNIROUEN) ; Normandie Université (NU)-Normandie Université (NU)-Université Le Havre Normandie (ULH) ; Normandie Université (NU)-CHU Rouen ; Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) ; Protéines de défense des réponses immune et inflammatoire : identification, régulation et rôles physiopathologiques ; Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM) ; Neuroendocrinologie cellulaire et moléculaire |
Link: | |
Zeitschrift: | PROTEOMICS, Jg. 6 (2006-05-31), S. 3926-3937 |
Veröffentlichung: | Wiley, 2006 |
Medientyp: | unknown |
ISSN: | 1615-9853 (print) ; 1615-9861 (print) |
DOI: | 10.1002/pmic.200500714 |
Schlagwort: |
|
Sonstiges: |
|