Murine DEP-1, a receptor protein tyrosine phosphatase, is expressed in macrophages and is regulated by CSF-1 and LPS
In: Journal of Leukocyte Biology, Jg. 64 (1998-11-01), S. 692-701
Online
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Zugriff:
The spectrum of protein tyrosine phosphatases (PTPs) expressed in bone marrow-derived murine macrophages (BMMs) was examined using reverse transcriptase-polymerase chain reaction. Ten different PTP cDNAs were isolated and in this study we focus on mDEP-1, a type III receptor PTP. Three mDEP-1 transcripts were expressed in primary macrophages and macrophage cell lines and were induced during macrophage differentiation of M1 myeloid leukemia cells. A variant mRNA was identified that encodes an alternate carboxyl-terminus and 3 UTR. The expression of mDEP-1 was down-regulated by CSF-1 (macrophage colony-stimulating factor) and up-regulated by bacterial lipopolysaccharide, an important physiological regulator of macrophage function that opposes CSF-1 action. Whole mount in situ hybridization, and immunolocalization of the protein, confirmed that mDEP-1 is expressed by a subset of embryonic macrophages in the liver and mesenchyme. mDEP-1 was also detected in the eye and peripheral nervous system of the developing embryo. Attempts to express mDEP-1 constitutively in the macrophage cell line RAW264 were unsuccessful, with results suggesting that the gene product inhibits cell proliferation. J. Leukoc. Biol. 64: 692–701; 1998.
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Murine DEP-1, a receptor protein tyrosine phosphatase, is expressed in macrophages and is regulated by CSF-1 and LPS
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Autor/in / Beteiligte Person: | J M Osborne ; Lichanska, Agnieszka M. ; N den Elzen ; Hume, David A. ; Cassady, A. I. ; Yamada, T ; Costelloe, Elaine O. |
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Zeitschrift: | Journal of Leukocyte Biology, Jg. 64 (1998-11-01), S. 692-701 |
Veröffentlichung: | Oxford University Press (OUP), 1998 |
Medientyp: | unknown |
ISSN: | 1938-3673 (print) ; 0741-5400 (print) |
DOI: | 10.1002/jlb.64.5.692 |
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