Antisense LNA-loaded nanoparticles of star-shaped glucose-core PCL-PEG copolymer for enhanced inhibition of oncomiR-214 and nucleolin-mediated therapy of cisplatin-resistant ovarian cancer cells

Vandghanooni, Somayeh ; Omidi, Yadollah ; et al.

In: International Journal of Pharmaceutics, Jg. 573 (2020), S. 118729-118729

Online unknown

Zugriff:

We aimed to inhibit overexpressed oncomiR-214 in cisplatin (CIS)-resistant ovarian cancer (OC) and perform targeted therapy of sensitized cells using a novel polymeric drug delivery system (DDS). A system of nanoparticles (NPs) of star-shaped glucose-core polycaprolactone-polyethylene glycol (Glu-PCL-PEG) block copolymer containing cisplatin (CIS-PCL NPs) and locked nucleic acid (LNA) anti-miR-214 (LNA-PCL NPs) were prepared and anti-nucleolin aptamer was conjugated to the surface of prepared NPs to prepare Ap-CIS-PCL NPs and Ap-LNA-PCL NPs, respectively. The cancer-targeting ability of the NPs was confirmed and the CIS-resistant A2780 (A2780 R) cells were transfected with Ap-LNA-PCL NPs to inhibit oncomiR-214 and sensitize the cells to CIS. Next, the miR-214-inhibited cells were exposed to the Ap-CIS-NPs and the deracination efficiency of targeted DDS was evaluated. The oncomiR-214 in A2780 R cells were harnessed by Ap-LNA-PCL NPs, and nucleolin-mediated endocytosis of targeted polymeric DDSs containing CIS into miR-214-inhibited A2780 R cells caused enhanced apoptosis, which was further confirmed by apoptosis detection and evaluation of downstream genes expression. Targeted inhibition of miR-214 using the developed NPs containing LNA can decrease drug-resistant properties of cancer cells and may enhance the efficiency of targeted DDSs.

Titel:	Antisense LNA-loaded nanoparticles of star-shaped glucose-core PCL-PEG copolymer for enhanced inhibition of oncomiR-214 and nucleolin-mediated therapy of cisplatin-resistant ovarian cancer cells
Autor/in / Beteiligte Person:	Vandghanooni, Somayeh ; Omidi, Yadollah ; Barar, Jaleh ; Eskandani, Morteza
Link:	View record in OpenAIRE (Volltext) E-Journal im Bestand der UB Hagen? https://doi.org/10.1016/j.ijpharm.2019.118729
Zeitschrift:	International Journal of Pharmaceutics, Jg. 573 (2020), S. 118729-118729
Veröffentlichung:	Elsevier BV, 2020
Medientyp:	unknown
ISSN:	0378-5173 (print)
DOI:	10.1016/j.ijpharm.2019.118729
Schlagwort:	Polyesters Aptamer medicine.medical_treatment Oligonucleotides Pharmaceutical Science Antineoplastic Agents CHO Cells macromolecular substances 02 engineering and technology 030226 pharmacology & pharmacy Polyethylene Glycols Targeted therapy 03 medical and health sciences Cricetulus Drug Delivery Systems 0302 clinical medicine Cell Line, Tumor medicine Animals Humans Locked nucleic acid health care economics and organizations Ovarian Neoplasms Cisplatin Drug Carriers Chemistry technology, industry, and agriculture RNA-Binding Proteins Transfection Aptamers, Nucleotide Oligonucleotides, Antisense Phosphoproteins 021001 nanoscience & nanotechnology MicroRNAs Glucose Drug Resistance, Neoplasm Apoptosis Cancer cell Cancer research Nanoparticles Female 0210 nano-technology Nucleolin medicine.drug
Sonstiges:	Nachgewiesen in: OpenAIRE Rights: CLOSED

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