Characterization of the long terminal repeats of micropia elements microdissected from the Y-chromosomal lampbrush loops 'threads' of Drosophila hydei
In: Journal of molecular biology, Jg. 209 (1989-10-05), Heft 3
Online
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Zugriff:
Four micropia elements from Drosophila melanogaster and D. hydei have been analysed by sequencing. Two elements, from D. hydei, micropia-DhMiF8 and -DhMiF2, were recovered by cloning microdissected Y-chromosomal lampbrush loops "threads". This method allows isolation of repetitive sequences from defined chromosomal positions, but recovery of large and overlapping inserts is difficult. In case of the Y-chromosomal micropia elements it was not possible to define the endpoints of their long terminal repeat sequences precisely. Comparison of these locus-defined micropia elements to complete micropia elements isolated from D. melanogaster allowed identification of micropia-DhMiF8 and micropia-DhMiF2 long terminal repeats (LTRs). LTR sequences from the two Drosophila species are not conserved except for a few short sequences found at comparable positions that are believed to have functional significance. In contrast, the Leu-tRNA primer binding site and plus strand primer binding site are conserved between D. melanogaster and D. hydei.
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Characterization of the long terminal repeats of micropia elements microdissected from the Y-chromosomal lampbrush loops 'threads' of Drosophila hydei
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Autor/in / Beteiligte Person: | Lankenau, Dirk-Henner ; Hennig, Wolfgang ; Huijser, Peter |
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Zeitschrift: | Journal of molecular biology, Jg. 209 (1989-10-05), Heft 3 |
Veröffentlichung: | 1989 |
Medientyp: | unknown |
ISSN: | 0022-2836 (print) |
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