Computational approach and electrochemical measurements for protein detection with MIP-based sensor
In: Biosensors and Bioelectronics Biosensors and Bioelectronics, Elsevier, 2020, 151, pp.111978. ⟨10.1016/j.bios.2019.111978⟩; (2020-03-01)
Online
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Zugriff:
International audience; Rapid and accurate detection of proteins in biological fluids is increasingly required in the biomedical environment. Actually, it is performed with conventional techniques, which are generally run by robotized platforms at centralized laboratories. In this work, molecular dynamics calculations and an experimental procedure were conducted to set up electrochemical sensors based on polypyrrol (PPy) molecular imprinted polymers (MIP) for proteins detection. Here, prostate-specific antigen (PSA) was selected as a template model. Computational calculations indicate that for any PPy conformation and any amino-acid location in the protein, PSA molecules remain strongly inserted in the PPy polymer without biological alterations. One from possible orientations, appeared to be most probable as it presents the lowest absorption energy (-363 kcal mol-1) and largest contact area (4034.1 Å2). The device was then elaborated by in situ electropolymerization of PPy films. MIP's thickness and extraction duration were optimized by chronoamperometry. Square wave voltammetry technique was investigated for PSA detection in standard solution in the concentration range of 3x10 -8 ng.ml-1- 300 ng ml-1. According to the Hill equation, the equilibrium dissociation constant Kdbetween PSA and its imprint was estimated at Kd = (1.02 ± 0.54) × 10-14 M, confirming the strong binding between the designed MIP and the protein as predicted by the computational study. PSA concentration values directly measured in 35 human serum samples were found closely correlated to those measured by the ELISA technique. The promising fast and low-cost sensor might be used successfully for proteins detection at low concentrations with high selectivity and reproducibility.
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Computational approach and electrochemical measurements for protein detection with MIP-based sensor
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Autor/in / Beteiligte Person: | Mazouz, Zouhour ; Kalfat, Rafik ; Othmane, Ali ; Omezzine, Asma ; Seydou, Mahamadou ; Yaakoubi, Nourdin ; Barbault, Florent ; Mokni, Meriem ; Zerrouki, Chouki ; Fourati, Najla ; Bouslema, Ali ; Institut National de Recherche et d'Analyse Physico-Chimique (INRAP) ; Institut National de Recherche et d'Analyse Physico-chimique (Ariana, Tunisie) (INRAP) ; Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE) ; École normale supérieure - Rennes (ENS Rennes)-Université Paris-Sud - Paris 11 (UP11)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-Université de Cergy Pontoise (UCP) ; Université Paris-Seine-Université Paris-Seine-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Cachan (ENS Cachan)-Université Gustave Eiffel (UNIV GUSTAVE EIFFEL) ; Hôpital Universitaire Sahloul (CHU Sahloul) ; Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS (UMR_7086)) ; Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7) ; Laboratoire d'Acoustique de l'Université du Mans (LAUM) ; Centre National de la Recherche Scientifique (CNRS)-Le Mans Université (UM) ; Laboratoire de biophysique ; Faculté de Médecine de Monastir [Tunisie] ; Laboratoire de recherche Méthodes et Techniques d'Analyses [Ariana] (LMTA) ; Institut National de Recherche et d'Analyse Physico-chimique [Ariana, Tunisie] (INRAP) ; Laboratoire des Interfaces et Matériaux Avancés [Monastir] (LIMA) ; Faculté des Sciences de Monastir (FSM) ; Université de Monastir - University of Monastir (UM)-Université de Monastir - University of Monastir (UM) ; École normale supérieure - Rennes (ENS Rennes)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Ecole Normale Supérieure Paris-Saclay (ENS Paris Saclay)-Université Gustave Eiffel-CY Cergy Paris Université (CY) ; Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP) |
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Quelle: | Biosensors and Bioelectronics Biosensors and Bioelectronics, Elsevier, 2020, 151, pp.111978. ⟨10.1016/j.bios.2019.111978⟩; (2020-03-01) |
Veröffentlichung: | HAL CCSD, 2020 |
Medientyp: | unknown |
ISSN: | 0956-5663 (print) |
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