The CREB Regulated Transcription Coactivator 2 Suppresses HIV-1 Transcription by Preventing RNA Pol II from Binding to HIV-1 LTR
In: Virologica Sinica, Jg. 36 (2021-03-15), S. 796-809
Online
unknown
Zugriff:
The CREB-regulated transcriptional co-activators (CRTCs), including CRTC1, CRTC2 and CRTC3, enhance transcription of CREB-targeted genes. In addition to regulating host gene expression in response to cAMP, CRTCs also increase the infection of several viruses. While human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) promoter harbors a cAMP response element and activation of the cAMP pathway promotes HIV-1 transcription, it remains unknown whether CRTCs have any effect on HIV-1 transcription and HIV-1 infection. Here, we reported that CRTC2 expression was induced by HIV-1 infection, but CRTC2 suppressed HIV-1 infection and diminished viral RNA expression. Mechanistic studies revealed that CRTC2 inhibited transcription from HIV-1 LTR and diminished RNA Pol II occupancy at the LTR independent of its association with CREB. Importantly, CRTC2 inhibits the activation of latent HIV-1. Together, these data suggest that in response to HIV-1 infection, cells increase the expression of CRTC2 which inhibits HIV-1 gene expression and may play a role in driving HIV-1 into latency.
Titel: |
The CREB Regulated Transcription Coactivator 2 Suppresses HIV-1 Transcription by Preventing RNA Pol II from Binding to HIV-1 LTR
|
---|---|
Autor/in / Beteiligte Person: | Cen, Shan ; Li, Xiaoyu ; Wang, Zhen ; Ma, Ling ; Guo, SaiSai ; Liu, Zhenlong ; Liang, Chen ; Ding, Jiwei ; Jia, Pingping ; Zhao, Jianyuan ; Guo, Fei ; Chen, Shumin ; Yi, Dongrong ; Li, Quanjie |
Link: | |
Zeitschrift: | Virologica Sinica, Jg. 36 (2021-03-15), S. 796-809 |
Veröffentlichung: | Elsevier BV, 2021 |
Medientyp: | unknown |
ISSN: | 1995-820X (print) ; 1674-0769 (print) |
DOI: | 10.1007/s12250-021-00363-1 |
Schlagwort: |
|
Sonstiges: |
|