Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial Virus
In: PloS one, Jg. 11 (2016-02-28), Heft 4
Online
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Zugriff:
Sensitive and precise serology assays are needed to measure the humoral response to antigens of respiratory syncytial virus (RSV) following natural infection or vaccination. We developed and evaluated a collection of electrochemiluminescent (ECL) serology assays using four RSV antigens (F, N, Ga and Gb). To assess the merits of ECL technology, the four ECL serology assays were evaluated using a well-characterized "gold standard" panel of acute and convalescent serum samples from fifty-nine RSV-positive and thirty RSV-negative elderly subjects (≥65 years old). The combined results from the four ECL assays demonstrated good concordance to the "gold standard" diagnosis, reaching 95% diagnostic sensitivity and 100% diagnostic specificity. Additionally, a combination of ECL assays provided higher diagnostic sensitivity than a commercially available diagnostic ELISA or cell-based microneutralization assay. In summary, these data demonstrate the advantages of using ECL-based serology assays and highlight their use as a sensitive diagnostic approach to detect recent RSV infection in an elderly population.
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Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial Virus
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Autor/in / Beteiligte Person: | Chu, Marla ; Zuo, Fengrong ; Ro, Bodrey ; Maifeld, Sarah V. ; Yamagata, Ryan ; Machhi, Shushil ; Parhy, Bandita ; Esser, Mark T. ; Ulbrandt, Nancy ; Falsey, Ann R. ; Walsh, Edward E. ; Chio, Vera ; Carlson, Marcia ; Mok, Hoyin ; C. Kathy Wang ; Yu, Li ; Park, Samuel ; Leonardson, Tansy |
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Zeitschrift: | PloS one, Jg. 11 (2016-02-28), Heft 4 |
Veröffentlichung: | 2016 |
Medientyp: | unknown |
ISSN: | 1932-6203 (print) |
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