RSC Chromatin-Remodeling Complex Is Important for Mitochondrial Function in Saccharomyces cerevisiae
In: PLoS ONE, Jg. 10 (2015-06-01), Heft 6
Online
unknown
Zugriff:
RSC (Remodel the Structure of Chromatin) is an ATP-dependent chromatin remodeling complex essential for the growth of Saccharomyces cerevisiae. RSC exists as two distinct isoforms that share core subunits including the ATPase subunit Nps1/Sth1 but contain either Rsc1or Rsc2. Using the synthetic genetic array (SGA) of the non-essential null mutation method, we screened for mutations exhibiting synthetic growth defects in combination with the temperature-sensitive mutant, nps1-105, and found connections between mitochondrial function and RSC. rsc mutants, including rsc1Δ, rsc2Δ, and nps1-13, another temperature-sensitive nps1 mutant, exhibited defective respiratory growth; in addition, rsc2Δ and nps1-13 contained aggregated mitochondria. The rsc2Δ phenotypes were relieved by RSC1 overexpression, indicating that the isoforms play a redundant role in respiratory growth. Genome-wide expression analysis in nps1-13 under respiratory conditions suggested that RSC regulates the transcription of some target genes of the HAP complex, a transcriptional activator of respiratory gene expression. Nps1 physically interacted with Hap4, the transcriptional activator moiety of the HAP complex, and overexpression of HAP4 alleviated respiratory defects in nps1-13, suggesting that RSC plays pivotal roles in mitochondrial gene expression and shares a set of target genes with the HAP complex.
This study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan to ET (22580086), and MY (19780076); URL: http://www.jsps.go.jp/j-grantsinaid/index.html.
Titel: |
RSC Chromatin-Remodeling Complex Is Important for Mitochondrial Function in Saccharomyces cerevisiae
|
---|---|
Autor/in / Beteiligte Person: | Chihara, Yuhki ; Nakamura, Misaki ; Yu, Feifei ; Hamada, Ryoko ; Imamura, Yuko ; Yukawa, Masashi ; Sato, Masahiro ; Okane, Kyo ; Tsuchiya, Eiko ; Kanai, Muneyoshi ; Ueno, Masaru |
Link: | |
Zeitschrift: | PLoS ONE, Jg. 10 (2015-06-01), Heft 6 |
Veröffentlichung: | Public Library of Science, 2015 |
Medientyp: | unknown |
ISSN: | 1932-6203 (print) |
Schlagwort: |
|
Sonstiges: |
|