A protein isolate from Moringa oleifera leaves has hypoglycemic and antioxidant effects in Alloxan-induced diabetic mice
In: Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules; Volume 22; Issue 2; Pages: 271 Molecules, Jg. 22 (2017), Heft 2, p 271
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Zugriff:
Moringa oleifera has been used in traditional medicine to treat diabetes. However, few studies have been conducted to relate its antidiabetic properties to proteins. In this study, a leaf protein isolate was obtained from M. oleifera leaves, named Mo-LPI, and the hypoglycemic and antioxidant effects on alloxan-induced diabetic mice were assessed. Mo-LPI was obtained by aqueous extraction, ammonium sulphate precipitation and dialysis. The electrophoresis profile and proteolytic hydrolysis confirmed its protein nature. Mo-LPI showed hemagglutinating activity, cross-reaction with anti-insulin antibodies and precipitation after zinc addition. Single-dose intraperitoneal (i.p.) administration of Mo-LPI (500 mg/kg·bw) reduced the blood glucose level (reductions of 34.3%, 60.9% and 66.4% after 1, 3 and 5 h, respectively). The effect of Mo-LPI was also evidenced in the repeated dose test with a 56.2% reduction in the blood glucose level on the 7th day after i.p. administration. Mo-LPI did not stimulate insulin secretion in diabetic mice. Mo-LPI was also effective in reducing the oxidative stress in diabetic mice by a decrease in malondialdehyde level and increase in catalase activity. Mo-LPI (2500 mg/kg·bw) did not cause acute toxicity to mice. Mo-LPI is a promising alternative or complementary agent to treat diabetes.
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A protein isolate from Moringa oleifera leaves has hypoglycemic and antioxidant effects in Alloxan-induced diabetic mice
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Autor/in / Beteiligte Person: | Talita Cavalcante Morais ; Martônio Ponte Viana ; Ana Fontenele Urano Carvalho ; Jose Tadeu Abreu de Oliveira ; Paulo Carvalho de Paula ; Daniele O.B. Sousa ; Santos, Flávia A. ; Bella Giselly Torres Alves ; Vasconcelos, Ilka M. ; Davi Felipe Farias ; Mirella Leite Pereira |
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Zeitschrift: | Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules; Volume 22; Issue 2; Pages: 271 Molecules, Jg. 22 (2017), Heft 2, p 271 |
Veröffentlichung: | Molecules, 2017 |
Medientyp: | unknown |
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