Total volatile basic nitrogen (TVBN), biogenic amine, total viable count (TVC), volatile compounds, and sensory evaluation were conducted to assess the quality of Chinese Mitten Crabs (Eriocheir sinensis) at living, zero, 2, 5, 10, 15, and 24 hr postmortem. The sensory evaluation found a noticeable odor of spoilage becoming evident 10 hr postmortem. The TVBN value increased and then decreased as time increased, reaching 23.67 mg N/100 g at 24 hr postmortem. Although biogenic amines were detected at 5 hr postmortem, by 24 hr postmortem these had not reached dangerous levels of toxicity. The initial TVC (6.06 Log CFU/g) of the living crab samples was relatively high and climbed further postmortem, reaching 10.00 Log CFU/g 24 hr postmortem. Trimethylamine was detected in the living sample in belly meat and 2 hr postmortem in crab roe and reached 8.33 µg/g in the roe 24 hr postmortem. Indole was detected at 0 hr (belly meat) and 10 hr (crab roe) postmortem, but did not change significantly during the observation period. Sulfur‐containing compounds were detected 5 hr after death and gradually increased over the observation period. Most indicators showed major changes at 5 hr and 10 hr postmortem. By 10 hr postmortem, the crab had entered the putrefaction stage and was thus no longer safe for consumption.
Keywords: chemical analysis; Chinese Mitten Crab (Eriocheir sinensis); flavor; putrefaction; quality assurance
This is the first research on the quality of Chinese Mitten crab at living and postmortem stage to the best of our knowledge. Quality indicators for the crab of early postmortem were investigated. A safe consumption timeline for Chinese Mitten crab has been suggested.
Chinese mitten crab (Eriocheir sinensis) is a traditional food popular in China and has been spread as an invasive species and found in Europe and North America as well. It has a unique, pleasant aroma, and delicate flavor. According to the China Fishery Yearbook (FBMA, [
Previous studies have generally focused on the quantification of the sensory score, K values, total volatile basic nitrogen (TVBN), biogenic amines, and total viable count (TVC) of bacteria and volatile compounds (Huidobro, López‐Caballero, & Mendes, [
Large amounts of biogenic amines, especially histamine, putrescine, cadaverine, and tyramine, can cause allergy‐like symptoms and food poisoning after ingestion (Hwang, Chang, Shiau, & Cheng, [
There have been studies on the influence of various treatments on Chinese mitten crab shelf life and quality, but few on the temporal characteristics of spoilage in untreated crab. As the traditional Chinese folk wisdom forbids eating crab that has been dead overnight, our objective was to establish characteristic indexes for the quality of Chinese mitten crab from a living state to the early stages postmortem. The current study examines what happens in crab samples that have died naturally, focusing on how five aspects of crab quality are affected by length of time postmortem, namely processing characteristics, sensory, physical, chemical, and microbiological factors. We hope that our results will provide a useful theoretical basis for improving the quality and safety of mitten crab products for human consumption.
A total of 60 female Chinese mitten crabs (average initial weight 137.79 ± 14.62 g) were harvested in October 2014 from a local processing company in Shanghai (Song Jiang) and confined in a cultivation cabinet at a constant temperature (20℃) and humidity (90%) until natural death occurred. The death rate of the crabs was calculated daily. Different states of the crab, namely living and 0, 2, 5, 10, 15, and 24 hr postmortem were sampled for this study at 20℃. The crab was steamed at 100℃ for 20 min (Chen, Zhang, & Shrestha, [
"Death" was determined once the animal's legs drooped naturally, and the eyes did not move when the animal was lifted by hand. The death rate (DR) was calculated as [DR (%) = (number of dead crabs/initial number of crabs) × 100].
The weight loss rate (WLR) was calculated as [WLR (%) = (weight loss/initial weight of crab) × 100].
The belly meat yield (MY) was calculated as [MY (%) = (weight of meat yield/weight of whole animal) × 100].
The crab roe yield (RY) was calculated as [RY (%) = (weight of crab roe/weight of whole animal) × 100].
Ten trained panelists, five male and five female, ranging in age from 20 to 25 years, performed a sensory assessment of the uncooked crab quality according to ISO [
1 TABLEScale used for Chinese mitten crab sensory evaluation
Description Best (5 point) Better (4 point) Middle (3 point) Worse (2 point) Worst (1 point) Crabs with unopened shells Color and luster Grayish with shine Darker gray with shine Grayish‐black with shine Quite black with less shine Crabs with unopened shells Odor Natural smell very strong Natural smell strong Natural smell light, off‐odor slightly No natural smell, smelly or ammonia smell Strong stench or ammonia smell Crabs with opened shells Organizational Structure Hepatopancreas and gonad tissue very tight Hepatopancreas and gonad tissue tight Hepatopancreas and gonad tissue less tight Hepatopancreas and gonad tissue loose Crabs with opened shells
TVBN was determined according to the method recommended by the European Commission Regulation ([
Biogenic amines were extracted and analyzed according to Özogul, Taylor, Quantick, and Özogul ([
Crab meat (3.000 g) was homogenized in 10 ml of 0.4 M perchloric acid (PCA) for 2 min and centrifuged at 5,000 r/min for 15 min at 4℃. After centrifugation, the upper layer was decanted and transferred into a 25 ml brown flask and diluted. A 1 ml extraction was derivatizated by 100 µl of 2 mol/L NaOH, 300 µl of a saturated solution of NaHCO
Total viable counts (TVC) for the uncooked meats were analyzed as per the technique described by Zhou, Liu, Xie, and Wang ([
A 65 µm carbowax/polydimethylsiloxane (CAR/PDMS) coated fused silica fiber was used as the solid phase. The coated fiber was inserted into a 20 ml headspace glass containing 2 g of homogenized sample and placed in a 90℃ water bath for 50 min. After adsorption, the fiber was immediately inserted into the GC–MS injector for 5 min and then removed.
An Agilent 6890 GC‐5975 mass selective detector (MSD) equipped with a DB‐5MS column (60 m length × 0.32 mm i.d. × 1 μm film thickness; Agilent Inc.) was used for the analysis. The initial oven temperature was 50℃ (no hold), followed by a 5℃/min linear ramp to 180℃ (no hold), and then an 8℃/min linear ramp to 240℃ (5 min hold). Helium (99.999%) was used as the carrier gas at a flow rate of 1 ml/min. The detector interface temperature was 250°C, the ion source temperature was 230°C, the ionization energy was 70 eV, the mass range was 40–450 a.m.u., the electron multiplier voltage was 1,576 V, and the scan rate was 1.8 s
Volatiles were intendified by mass spectra matching (Wiley/NIST 2008 database) with compound matching greater than 800. The quantitation of compounds was according to the rentention index (RI).
RI was calculated as follows:
where Rt(x) is the retention time of each volatile compound (x). Rt(n) and Rt(n + 1) are the retention times of the n‐alkanes eluting directly before and after the compound (x) under identical chromatographic conditions.
For quantification, 2,4,6‐trimethyl pyridine (TMP) was used as an internal standard, with 1 µg introduced into each 2 g homogenized sample.
The concentration of each volatile compound (Conc) was calculated as follows:
All measurements were conducted in triplicate. The data are presented as mean ± standard deviation (SD). SPSS 19.0 (SPSS Inc., Chicago, IL, USA) was used for the statistical analyses of the various indicators. Analysis of variance (ANOVA) was performed on the group means of the crab meat samples, and significant differences were tested at p ≤ .05. Changes in the meat content of each sample were mapped using SigmaPlot 12.5 (Systat Software Inc.) software.
The crabs died at varying rates as the storage time progressed, with the death rate being particularly high in the first three days. After storage in the cultivation cabinet at 20 ± 1℃ for 1 day, 28.33% of the crabs were dead; after 4 days, 70.00% of the crabs had died. The rate slowed thereafter, but all the crabs were dead by day 10 (Figure 1).
The quality of the living crabs was considered the "initial quality," with a weight loss rate of 0. The crab weight loss rate increased steeply to 8.18% by 24 hr postmortem (Figure 2), with the edible parts yield of E. sinensis decreasing as the postmortem period increased. The yield for living crabs was generally similar to that reported by Wu et al. ([
Figure 3 shows the trends in the sensory evaluation scores of the Chinese mitten crabs as time progressed. The sensory scores declined as postmortem time increased. The shells of the living crabs were grayish and glossy, and they had well‐organized roe with a distinct, fresh odor, and no "off‐flavors." As the length of time postmortem (or "death time") increased, the crab shell gradually turned black and dull, while the roe structure began to loosen. By 24 hr postmortem, the crabs were rotting and producing a pungent, ammonia‐like odor. The layer of mucous membrane below the heart had become blurred and opaque. The crab samples' sensory scores declined most rapidly in the first 5 hr postmortem. A strong odor was present by 10 hr postmortem, at which point the overall color score was 3.07, the odor score was 2.01, and the opened shells' organizational structure score was 3.48. Rejection at this point was attributed to the pronounced ammoniac odor, although the crab paste inside the shell was still relatively well organized; the score for odor was lower than the overall score for shell color or paste inside the shell (Figure 3). These results demonstrate that the sensory score is primarily influenced by odor, with the other factors playing only a secondary role.
The TVBN value, a common indicator of spoilage, increased and then decreased as death time increased, which differs from the trends reported for other seafood products such as fish or shrimp. Previous studies on wild white grouper (Özogul, Özogul, & Kuley, [
The European Commission has set the legal limit for TVBN in seafood in the range from 25 to 35 mgN/100 g (EC, [
Table 2 shows the biogenic amine content of the Chinese mitten crab samples at different times postmortem. The biogenic amines found in crab mainly consist of putrescine, cadaverine, tyramine, and small amounts of spermidine and spermine. In previous studies, histamine—an important indicator of aquatic toxicity and hygienic quality—has been found to be present even immediately after catching the animal (Lehane & Olley, [
2 TABLEChanges in the amount of biogenic amine extracted from crabs in different states
State Putrescine Cadaverine Tyramine Spermidine Spermine BM CR BM CR BM CR BM CR BM CR D5 6.82 ± 2.04a N.D. N.D. N.D. 3.76 ± 0.85a N.D. N.D. N.D. N.D. N.D. D10 15.06 ± 2.04b 9.14 ± 2.60a 4.33 ± 1.15a 7.40 ± 0.52a 4.02 ± 0.75a 6.59 ± 0.98a 3.09 ± 0.60a N.D. N.D. 3.59 ± 1.03a D15 102.45 ± 9.04c 146.78 ± 13.62b 15.10 ± 2.81b 10.03 ± 3.38b 9.45 ± 2.10b 8.37 ± 1.22a N.D N.D. 3.59 ± 1.03a 3.05 ± 1.21a D24 197.72 ± 11.21d 218.66 ± 9.83c 25.71 ± 4.17c 27.84 ± 2.36c 21.42 ± 6.51c 27.59 ± 5.81b 2.97 ± 0.77a 3.15 ± 0.27a 4.01 ± 0.76a 5.25 ± 0.76b
1 Note
- 3 Values are presented as means ± SD. Unit: mg/kg. Three replications tested. D5, D10, D15, and D24 indicate 5, 10, 15, and 24 hr postmortem, respectively.
- 4 Abbreviations: BR, belly meat; CR, crab roe.
- 5 Different superscript letters in the same column indicate a significant difference p < .05.
No biogenic amines were detected in the living, 0 hr, or 2 hr dead crab samples. The amount of putrescine, cadaverine, and tyramine increased from 5 hr postmortem onwards, with the putrescine content increasing faster than either cadaverine or tyramine. This may be related to the content of amino acids and the precursors of putrescine, cadaverine, and tyramine: ornithine, lysine, and tyrosine, respectively (Anacleto et al., [
The putrescine, cadaverine, and tyramine content in the belly meat reached 197.72 mg/100 g, 25.71 mg/100 g, and 21.42 mg/100 g and in the roe reached 218.66 mg/100 g, 27.84 mg/100 g, and 27.59 mg/100 g, respectively, at 24 hr postmortem (Table 2). None of the levels of biogenic amines were sufficient to cause poisoning; the histamine limit in food is 50 mg/kg (FDA), and the LD50 (median lethal dose) for putrescine and cadaverine are 1,750 mg/kg and 270 mg/kg, respectively (Oguri et al., [
The initial TVC (6.06 Log CFU/g) of the living crab samples (Figure 5) represented a very high background content compared with those reported for raw snow crab (2.5 Log CFU/g), raw blue crab meat (2.5 Log CFU/g), or fresh puffer fish (2.80 Log CFU/g) (Lorentzen et al., [
TVC increased quickly immediately postmortem (6.62 Log CFU/g) to 5 hr postmortem (8.90 Log CFU/g) and then continued to increase slowly until it reached 10.00 Log CFU/g at 24 hr postmortem. The sudden increase in microbial growth at death corresponds closely to the belly meat yield and sensory evaluation results. (Özogul, Polat, & Özogul, [
Sixty‐six volatile compounds were identified in the belly meat and roe in different states (Table 3). These were 23 hydrocarbons, 11 alcohols, 4 ketones, 17 aldehydes, 5 N‐containing compounds, 4 S‐containing compounds, and 2 "others." Among the 66 volatile compounds, 47 were found in the belly meat and 48 in the roe.
3 TABLEChanges in the content of volatile compounds extracted from crabs in different states
Number Compounds Means of identification Belly meat Crab roe A D0 D2 D5 D10 D15 D24 A D0 D2 D5 D10 D15 D24 Hydrocarbons W1 Undecane MS,RI 144.92 ± 16.9 75.12 ± 9.06 43.37 ± 10.83 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W2 Decane, 1,1'‐oxybis‐ MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. 76.82 ± 15.84 52.89 ± 46.2 N.D. N.D. N.D. N.D. N.D. W3 Dodecane MS,RI 708.06 ± 91.96 624.29 ± 23.53 427.81 ± 17.73 210.63 ± 26.29 116.84 ± 10.03 85.28 ± 29.13 68.78 ± 10.60 1,270.00 ± 205.69 892.45 ± 28.55 824.25 ± 150.88 727.79 ± 157.29 633.60 ± 120.52 428.42 ± 15.0.39 345.78 ± 32.49 W4 1‐Tridecene MS,RI 135.66 ± 18.16 104.38 ± 19.17 60.29 ± 9.86 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W5 Tridecane MS,RI 1,242.10 ± 111.44 1,067.83 ± 99.51 852.38 ± 101.00 537.29 ± 86.52 231.77 ± 11.67 184.64 ± 21.43 121.77 ± 14.28 N.D. N.D. N.D. N.D. N.D. N.D. N.D. W6 Heptylcyclohexane MS 101.46 ± 12.51 87.39 ± 13.02 64.72 ± 14.34 43.37 ± 17.39 N.D. 68.38 ± 10.31 83.26 ± 9.42 89.04 ± 13.35 132.23 ± 50.3 N.D. N.D. 44.32 ± 3.52 63.82 ± 6.37 99.95 ± 4.51 W7 Tetradecane, 4‐methyl MS,RI 141.93 ± 16.02 135.27 ± 9.83 86.49 ± 20.17 63.28 ± 8.47 24.49 ± 6.31 85.31 ± 6.35 165.38 ± 15.39 116.44 ± 25.25 N.D. N.D. N.D. 61.63 ± 5.17 102.38 ± 8.05 164.24 ± 9.94 W8 Tridecane, 3‐methylene‐ MS,RI 44.73 ± 5.53 20.36 ± 7.73 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W9 3‐Tetradecene, (E)‐ MS,RI 112.31 ± 13.66 79.37 ± 12.58 27.69 ± 10.03 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W10 Tetradecane MS 339.16 ± 10.37 233.27 ± 27.51 N.D. 103.38 ± 9.23 71.95 ± 10.56 53.31 ± 17.39 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W11 Tetradecane, 2,6,10‐trimethyl MS,RI 68.73 ± 1.09 127.31 ± 5.26 28.65 ± 2.94 N.D. N.D. N.D. N.D. 73.55 ± 17.74 121.76 ± 47.41 97.88 ± 4.72 N.D. N.D. 103.28 ± 13.92 87.57 ± 1.86 W12 Hexadecane, 2,6,10,14‐tetramethyl‐ MS,RI 227.37 ± 32.67 349.29 ± 17.42 265.72 ± 25.16 187.21 ± 14.37 121.5 ± 19.53 74.51 ± 20.15 36.29 ± 8.47 N.D. N.D. N.D. N.D. N.D. N.D. N.D. W13 1‐Pentadecene MS,RI 206.74 ± 10.96 164.52 ± 20.27 121.92 ± 13.41 62.38 ± 15.62 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W14 Pentadecane MS,RI 178.15 ± 10.35 132.47 ± 12.38 154.27 ± 7.25 100.43 ± 17.21 85.41 ± 34.75 N.D. 47.52 ± 18.06 478.44 ± 44.52 265.63 ± 129.18 397.71 ± 38.69 361.76 ± 25.41 98.35 ± 12.24 136.59 ± 9.58 372.08 ± 42.79 W15 n‐Nonylcyclohexane MS,RI 234.12 ± 7.52 234.12 ± 7.53 234.12 ± 7.54 234.12 ± 7.55 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W16 Pentadecane, 3‐methyl‐ MS,RI 129.98 ± 8.87 64.21 ± 5.42 42.59 ± 8.25 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W17 Hexadecane MS,RI 268.90 ± 18.24 136.42 ± 12.31 98.99 ± 2.86 69.20 ± 14.29 59.58 ± 13.39 36.57 ± 8.51 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W18 Cyclopentane, undecyl‐ MS,RI 108.79 ± 5.97 78.63 ± 8.67 97.35 ± 11.70 67.42 ± 7.12 35.27 ± 2.07 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W19 1‐Eicosene MS,RI 66.67 ± 10.42 43.66 ± 8.26 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W20 Pentadecane, 2,6,10,14‐tetramethyl‐ MS 1,377.32 ± 118.09 1,028.52 ± 72.91 1,472.01 ± 114.27 1,007.32 ± 92.32 948.6 ± 170.89 872.81 ± 189.02 664.02 ± 52.14 2,914.88 ± 1,337.25 2,338.44 ± 1,182.11 2,103.31 ± 81.6 2003.31 ± 81.6 1875.23 ± 152.04 1745.17 ± 102.47 1675.91 ± 92.4 W21 Heptacosane MS,RI 49.04 ± 13.23 143.81 ± 16.84 148.92 ± 18.43 103.93 ± 9.41 95.46 ± 9.44 63.65 ± 8.92 54.21 ± 10.37 N.D. N.D. N.D. N.D. N.D. N.D. N.D. W22 Cyclohexane, undecyl‐ MS,RI 59.00 ± 5.41 20.62 ± 4.37 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. W23 Oxirane, hexadecyl‐ MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. 534.32 ± 39.63 N.D. 906.98 ± 110.81 906.98 ± 110.81 337.08 ± 62.9 N.D. N.D. Subtotal 5,945.14 ± 539.37 4,950.86 ± 413.58 4,227.29 ± 395.77 2,789.96 ± 325.79 1,790.87 ± 288.64 1524.46 ± 311.21 1,452.59 ± 113.92 5,033.67 ± 393.114 4,805.65 ± 338.34 4,007.74 ± 1644.46 3,980.95 ± 1,660.89 3,362.39 ± 268.36 3,079.66 ± 140.39 2,992.20 ± 180.05 Alcohol C1 1‐Octen‐3‐ol MS,RI N.D. N.D. N.D. 136.48 ± 12.52 62.04 ± 16.30 N.D. N.D. 216.55 ± 51.97 161.55 ± 96.92 N.D. 59.77 ± 34.51 N.D. N.D. N.D. C2 2‐Octen‐1‐ol MS N.D. N.D. N.D. N.D. N.D. N.D. N.D. 42.56 ± 17.47 N.D. N.D. N.D. N.D. N.D. N.D. C3 2‐Octyn‐1‐ol MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. 233.16 ± 144.12 36.58 ± 63.36 34.9 ± 15.86 N.D. N.D. N.D. N.D. C4 4‐nonanol MS,RI N.D. N.D. N.D. N.D. 208.8 ± 8.53 153.62 ± 10.26 83.61 ± 15.17 N.D. N.D. 129.06 ± 18.17 N.D. 58.22 ± 5.11 N.D. N.D. C5 3‐Decen‐1‐ol, (Z)‐ MS,RI N.D. 321.83 ± 23.47 251.82 ± 18.26 217.28 ± 24.51 183.11 ± 10.86 93.71 ± 16.27 61.35 ± 9.21 N.D. N.D. N.D. N.D. N.D. N.D. N.D. C6 10‐undecenol MS,RI N.D. N.D. N.D. N.D. 342.19 ± 39.21 210.52 ± 18.31 152.24 ± 17.36 N.D. 980.17 ± 85.75 1,351.56 ± 37.53 N.D. 748.05 ± 52.19 521.20 ± 15.10 221.72 ± 56.75 C7 6‐Pentadecen‐1‐ol, (Z)‐ MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 137.33 ± 22.02 N.D. N.D. N.D. C8 1‐Hexadecanol, 2‐methyl‐ MS,RI 129.40 ± 12.27 218.41 ± 17.28 153.92 ± 13.71 32.51 ± 17.28 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. C9 1‐Dodecanol, 3,7,11‐trimethyl‐ MS 50.48 ± 4.66 27.27 ± 2.34 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 24.82 ± 7.47 56.28 ± 5.63 68.48 ± 7.47 C10 3,7,11,15‐Tetramethyl‐2‐hexadecen‐1‐ol MS,RI 70.23 ± 8.4 73.01 ± 6.43 42.90 ± 7.31 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 20.06 ± 14.11 35.17 ± 5.72 N.D. C11 1‐Heptacosanol MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D 33.49 ± 2.46 26.92 ± 3.54 N.D. Subtotal 250.11 ± 25.33 640.52 ± 49.52 448.64 ± 39.28 386.27 ± 54.31 796.14 ± 74.9 457.85 ± 44.84 297.2 ± 41.74 492.27 ± 123.56 1,351.56 ± 37.53 1,351.56 ± 37.53 197.10 ± 56.53 985.74 ± 95.57 639.57 ± 29.99 290.2 ± 64.22 Ketone T1 2‐Butanone MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. 27.14 ± 5.79 19.82 ± 6.48 N.D N.D N.D N.D N.D T2 2‐Nonanone MS,RI N.D. N.D. N.D. N.D. 48.96 ± 10.64 24.91 ± 8.02 N.D. N.D. 92.14 ± 12.87 N.D. N.D. 53.52 ± 17.95 42.03 ± 7.42 N.D. T3 5,9‐Undecadien‐2‐one, 6,10‐dimethyl‐, (E)‐ 57.2 ± 11.24 86.27 ± 12.62 153.01 ± 16.39 62.03 ± 9.27 57.2 ± 11.24 86.27 ± 12.62 153.01 ± 16.39 N.D. N.D. N.D. N.D. N.D. N.D. N.D. T4 3‐Buten‐2‐one, 4‐(2,6,6‐trimethyl‐1‐cyclohexen‐1‐yl)‐ MS N.D. N.D. N.D. N.D. N.D. N.D. N.D. 78.08 ± 8.75 87.95 ± 25.44 N.D. N.D. N.D. N.D. N.D. Subtotal 57.2 ± 11.24 86.27 ± 12.62 153.01 ± 16.39 62.03 ± 9.27 90.65 ± 20.16 112.84 ± 19.63 153.01 ± 16.39 106.24 ± 14.91 200.01 ± 40.31 N.D. N.D. 53.52 ± 17.95 26.41 ± 6.52 N.D. Aldehyde . Q1 Benzaldehyde MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 237.06 ± 136.86 216.02 ± 9.83 231.87 ± 26.14 3.06 ± 1.76 15.79 ± 3.75 23.23 ± 3.04 Q2 Benzeneacetaldehyde MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. 80.60 ± 19.24 161.14 ± 62.07 164.58 ± 48.54 175.53 ± 23.28 56.70 ± 3.82 56.70 ± 3.83 104.83 ± 3.25 Q3 2‐Octenal MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. 77.10 ± 21.66 80.75 ± 37.43 N.D. N.D. 26.23 ± 2.34 N.D. N.D. Q4 Nonanal MS,RI 481.24 ± 50.53 637.38 ± 60.43 748.73 ± 58.79 497.38 ± 38.52 428.19 ± 34.79 206.47 ± 42.58 174.38 ± 32.38 372.37 ± 50.08 640.63 ± 163.22 N.D. N.D. 363.59 ± 29.67 257.15 ± 20.86 N.D. Q5 2,6‐Nonadienal MS N.D. N.D. N.D. N.D. N.D. N.D. N.D. 119.74 ± 25.91 137.04 ± 27.32 134.47 ± 9.5 121.77 ± 21.9 73.65 ± 1.36 52.17 ± 6.28 N.D. Q6 cis‐4‐Decenal MS,RI N.D. N.D. N.D. N.D. 52.15 ± 2.24 N.D. 50.97 ± 9.29 110.90 ± 38.3 115.86 ± 10.78 N.D. 51.63 ± 2.71 N.D. N.D. Q7 Decanal MS,RI 132.95 ± 2.84 157.58 ± 31.09 204.47 ± 22.21 187.31 ± 25.71 158.47 ± 22.21 85.53 ± 21.71 N.D. 115.29 ± 18.81 149.19 ± 47.54 215.82 ± 52.75 211.37 ± 75.44 93.42 ± 5.23 37.35 ± 4.17 N.D. Q8 2‐Decenal MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. 66.52 ± 31.04 N.D. N.D. N.D. 51.60 ± 8.11 N.D. N.D. Q9 Undecanal MS,RI 69.24 ± 4.13 87.29 ± 12.71 105.63 ± 18.42 145.48 ± 10.35 117.68 ± 12.35 75.42 ± 14.36 65.17 ± 8.54 78.75 ± 16.14 86.56 ± 18.36 111.80 ± 6.98 96.87 ± 9.13 84.59 ± 8.05 27.27 ± 4.26 N.D. Q10 2,4‐Decadienal MS N.D. N.D. N.D. N.D. N.D. N.D. N.D. 75.48 ± 18.87 N.D. N.D. N.D. N.D. N.D. N.D. Q11 2‐Undecenal MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. 138.14 ± 34.22 N.D. N.D. N.D. N.D. N.D. N.D. Q12 7‐Hexadecenal MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. 29.03 ± 8.19 N.D. N.D. N.D. N.D. N.D. N.D. Q13 Dodecanal MS,RI 41.04 ± 4.98 63.28 ± 8.02 71.93 ± 12.03 52.47 ± 6.43 34.62 ± 6.16 40.53 ± 12.42 64.22 ± 9.84 83.17 ± 10.87 74.85 ± 31.96 90.02 ± 9.5 79.93 ± 2.84 55.21 ± 5.82 54.68 ± 6.21 84.73 ± 53.63 Q14 Tridecanal MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. 93.09 ± 12.68 74.06 ± 51.06 N.D. 67.12 ± 7.63 33.58 ± 9.87 2036 ± 10.27 N.D. Q15 Tetradecanal MS,RI 45.70 ± 3.36 78.29 ± 8.62 104.83 ± 17.42 65.39 ± 6.27 36.06 ± 7.78 26.41 ± 3.52 N.D. 66.61 ± 4.64 156.86 ± 70.46 185.79 ± 3.48 126.98 ± 19.99 75.16 ± 11.84 60.26 ± 7.25 55.77 ± 11.12 Q16 Hexadecanal MS,RI 183.09 ± 4.32 317.26 ± 15.31 559.47 ± 23.18 486.19 ± 16.28 212.02 ± 66.93 196.53 ± 13.27 137.39 ± 27.64 624.51 ± 60.98 1,243.98 ± 81.04 3,795.4 ± 175.44 2,819.65 ± 294.04 1,434.83 ± 161.42 1,328.59 ± 92.25 1,278.34 ± 130.04 Q17 Octadecanal MS,RI 123.31 ± 12.32 167.93 ± 25.37 186.28 ± 18.02 97.29 ± 12.54 51.60 ± 13.01 63.38 ± 17.31 48.18 ± 9.27 306.89 ± 40.84 282.79 ± 239.18 1,078.57 ± 392.65 146.16 ± 128.38 219.73 ± 64.54 278.24 ± 14.52 321.94 ± 30.45 Subtotal 1,076.57 ± 82.48 1509.01 ± 161.55 2,134.35 ± 186.46 1593.54 ± 125.37 1,180.90 ± 179.01 758.91 ± 139.5 489.34 ± 87.67 2,378.26 ± 142.18 3,435.18 ± 256.24 5,642.80 ± 239.18 4,028.82 ± 392.65 2,622.98 ± 128.38 4,912.21 ± 217.58 1875.16 ± 123.61 N‐containing compounds N1 Trimethylamine MS,RI 427.42 ± 19.27 1,740.21 ± 42.52 4,145.94 ± 146.85 5,974.59 ± 217.45 6,386.93 ± 218.36 7,328.58 ± 416.48 8,328.48 ± 743.27 N.D. N.D. 142.61 ± 12.17 489.60 ± 40.27 942.74 ± 47.16 1549.64 ± 58.39 2,242.61 ± 52.17 N2 Oxime‐, methoxy‐phenyl‐ MS 366.66 ± 79.07 325.64 ± 39.38 312.39 ± 28.46 296.39 ± 24.82 291.53 ± 160.17 182.38 ± 37.31 112.58 ± 38.02 1,289.71 ± 44.78 1,135.71 ± 87.01 1,074.63 ± 53.15 879.71 ± 29.67 692.59 ± 44.78 702.46 ± 62.03 673.56 ± 66.60 N3 1‐Butanamine, N‐butyl‐ 496.24 ± 316.5 672.24 ± 69.36 536.82 ± 42.03 496.24 ± 316.8 595.91 ± 24.27 621.49 ± 52.06 548.28 ± 90.45 N.D. N.D. N.D. N.D. N.D. N.D. N.D. N4 Formamide, N,N‐dibutyl‐ MS N.D. N.D. N.D. N.D. N.D. N.D. N.D. 35.29 ± 1.78 113.42 ± 14.13 180.61 ± 6.6.8 N.D. 53.80 ± 2.14 75.54 ± 9.48 84.99 ± 7.46 N5 Indole MS N.D. 18.28 ± 3.93 22.64 ± 4.86 38.35 ± 8.75 86.79 ± 7.42 149.97 ± 10.36 328.47 ± 16.49 N.D. N.D. N.D. N.D. 23.61 ± 8.37 35.74 ± 5.54 49.17 ± 8.39 S‐containing compounds S1 Dimethyl sulfide MS,RI N.D. 35.73 ± 7.45 74.92 ± 9.43 123.04 ± 11.38 285.83 ± 14.70 402.84 ± 32.67 864.03 ± 120.36 N.D. N.D. N.D. 279.49 ± 17.39 292.57 ± 15.30 469.38 ± 20.31 800.37 ± 21.05 S2 Dimethyldisulfide MS N.D. N.D. N.D. 37.07 ± 3.85 86.43 ± 10.49 178.38 ± 26.07 386.64 ± 41.93 N.D. N.D. N.D. N.D. 72.52 ± 9.06 103.37 ± 12.48 255.39 ± 32.72 S3 Dimethyltrisulfide MS N.D. N.D. N.D. N.D. 42.86 ± 9.65 74.85 ± 6.47 121.58 ± 12.65 N.D. N.D. N.D. N.D. 37.58 ± 8.32 58.15 ± 10.32 85.46 ± 19.44 S4 Tetrasulfide, dimethyl MS,RI N.D. N.D. N.D. N.D. 28.73 ± 5.72 35.96 ± 6.74 47.93 ± 6.68 N.D. N.D. N.D. N.D. 14.80 ± 3.38 19.27 ± 4.92 24.74 ± 4.63 Others Z1 3‐(1‐Cyclopentenyl) furan MS,RI N.D. N.D. N.D. N.D. N.D. N.D. N.D. 54.83 ± 31.7 N.D. N.D. N.D. N.D. N.D. N.D. Z2 Butylated Hydroxytoluene MS,RI 144.09 ± 22.2 253.27 ± 17.39 336.71 ± 28.79 187.96.09 ± 14.83 90.39 ± 14.38 83.53 ± 16.93 63.07 ± 14.39 35.29 ± 1.78 113.42 ± 14.13 180.61 ± 6.6.8 N.D. 53.80 ± 2.14 63.28 ± 12.61 84.99 ± 7.46
- 2 Note
- 6 Values are presented as means ± SD. Unit: µg/kg. Three replications tested. A, D0, D2, D5, D10, D15, and D24 indicate alive, 0, 2, 5, 10, 15, and 24 hr postmortem, respectively.
- 7 In column 1: W stands for Alkanes, C stands for Alcohols, T stands for: Acetones, Q stands for: Aldehydes, N stands for: Nitrogen‐containing compounds, S stands for: Sulfur‐containing compounds and Z stands for: other compounds.
Hydrocarbons usually make only a minimal contribution to odor due to their high odor thresholds, and they only affect the flavor at relatively high contents (Gu et al., [
Due to their high odor thresholds, alcohols also usually contribute little to the overall flavor unless they are present at very high levels or contain unsaturated bonds. The alcohol volatility changed as time after death increased. Small molecular weight alcohols (1‐Octen‐3‐ol; 2‐Octen‐1‐ol; 2‐Octyn‐1‐ol) were identified in the roe within 5 hr postmortem, and large molecular weight alcohols (3,7,11‐trimethyl‐; 3,7,11,15‐Tetramethyl‐2‐hexadecen‐1‐ol; 1‐Heptacosanol) were identified in 10 hr postmortem. This trend was not observed in the belly meat, however. This may be associated with the production of alcohols caused by sugar degradation and fat oxidation.
A low ketone content was also identified: 2‐Nonanone was observed at 10 and 15 hr postmortem for belly meat, and 3‐Buten‐2‐one, 4‐(2,6,6‐trimethyl‐1‐cyclohexen‐1‐yl) was observed in living crab and 0 hr postmortem in the roe.
Aldehydes have low odor thresholds and typically form due to fat degradation, making them effective indicators of aroma in seafood products. Among the 17 aldehydes present in the samples, 7 were found in the belly meat and 17 in the roe. The content increased and then decreased with increasing time postmortem. For belly meat, cis‐4‐Decenal was only identified 10 hr postmortem and 2,4‐Decadienal, 2‐Undecenal, and 7‐Hexadecenal only in the living crab roe. These results may be associated with aldehyde production; amino acid Strecker reactions and lipid oxidative degradation are the two main sources of aldehydes in crustaceans (Baek & Cadwallader, [
N‐ and S‐containing compounds are also known to contribute to aquatic aromas. We found five N‐containing compounds in our samples, including TMA and indole. Pure TMA is associated with ammonia‐like odors that are not present in very fresh seafood. It is typically found together with acid and pyridine, which are responsible for "fishy" odors. Oxime‐ and methoxy‐phenyl‐, which are associated with mildewy and meaty odors, gradually decreased as death time increased; 1‐Butanamine and N‐butyl‐ were only found in the belly meat, while formamide and N,N‐dibutyl‐ were only detected in the roe.
TMA commonly serves as an indicator of spoilage in aquatic products. The content of TMA sharply increased as time after death increased. Other researchers have also reported this trend: TMA levels rose from 0.43 µg/g (in the living samples) to 8.33 µg/g (at 24 hr postmortem) for cooked blue crab stored at 4℃, 40.80 µg/g (at 8 days) and 30.00 µg/g (7 days) for wild white grouper stored on ice (Özogul et al., [
Indole has been used as an indicator of sepsis in shrimp (Snellings et al., [
S‐containing compounds, which are also associated with irritating odors, were identified at 5 hr postmortem in accordance with the sensory evaluation. These levels are closely related to s‐containing free amino acids, thiamine, and glutathione (Girard & Durance, [
In a series of Chinese mitten crab samples, the sensory evaluation scores decreased as time postmortem increased. A noticeable odor of spoilage was present by 10 hr postmortem. The TVBN value (23.67 mg N/100 g at 24 hr postmortem) increased and then decreased as time progressed. Biogenic amines were detected at 5 hr postmortem and 24 hr postmortem, but at relatively low (non‐poisonous) levels. Trimethylamine was detected in living crab belly meat and 2 hr postmortem (8.33 µg/g at 24 hr) in crab roe. Indole was detected at 0 hr (belly meat) and 10 hr (crab roe) postmortem, but did not change significantly throughout the observation period. Sulfur‐containing compounds were detected 5 hr postmortem and gradually increased as time postmortem increased. The samples did not exceed the legal limit for TVBN within the relatively brief storage period (24 hr), but still showed relatively high levels of biogenic amines and volatile compounds. These indicators suggest that the crab was already corrupted by 10 hr postmortem. Chinese mitten crabs that have been stored postmortem overnight are thus not recommended for consumption due to their unacceptable sensory evaluation scores and high TVC content. Future study may focus on different processing methods (steaming, frying) on the qualify aspects of the crab.
This work was supported by the National Natural Science Foundation of China (NSFC) (Projects No. 31471608 and No. 31471685).
The authors declare that they do not have any conflict of interest. This study does not involve any patients or animal testing.
By Yahui Wang; Yaozhou Zhu; Wenzheng Shi and Xichang Wang
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