Autologous immuno magnetically selected CD133+ stem cells in the treatment of no-option critical limb ischemia: clinical and contrast enhanced ultrasound assessed results in eight patients
In: Journal of Translational Medicine, Jg. 13, Heft 1
Online
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Zugriff:
Objectives Demonstrate the safety and effectiveness of highly purified CD133+ autologous stem cells in critical limb ischemia (CLI). Design Prospective single-center not randomized. Clinicaltrials.gov identifier: NCT01595776 Methods Eight patients with a history of stable CLI were enrolled in a period of 2 years. After bone marrow stimulation and single leukapheresis collection, CD133+ immunomagnetic cell selection was performed. CD133+ cells in buffer phosphate suspension was administered intramuscularly. Muscular and arterial contrast enhanced ultra sound (CEUS), lesion evolution and pain management were assessed preoperatively and 3, 6 and 12 months after the implant. Results No patient had early or late complications related to the procedure. Two patients (25 %) didn’t get any relief from the treatment and underwent major amputation. Six patients (75 %) had a complete healing of the wounds, rest pain cessation and walking recovery. An increase in CEUS values was shown in all eight patients at 6 months and in the six clinical healed patients at 12 months and had statistical relevance. Conclusions Highly purified autologous CD133+ cells can stimulate neo-angiogenesis, as based on clinical and CEUS data. Electronic supplementary material The online version of this article (doi:10.1186/s12967-015-0697-4) contains supplementary material, which is available to authorized users.
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Autologous immuno magnetically selected CD133+ stem cells in the treatment of no-option critical limb ischemia: clinical and contrast enhanced ultrasound assessed results in eight patients
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Autor/in / Beteiligte Person: | Perotti, Cesare ; Fabrizio, Calliada ; Moia, Alessia ; Ragni, Franco ; Bozzani, Antonio ; Pagani, Michele ; Claudia Del Fante ; Arici, Vittorio ; Alessandrino, Francesco ; Viarengo, Gianluca ; Tinelli, Carmine |
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Zeitschrift: | Journal of Translational Medicine, Jg. 13, Heft 1 |
Veröffentlichung: | Springer Nature |
Medientyp: | unknown |
ISSN: | 1479-5876 (print) ; 0159-5776 (print) |
DOI: | 10.1186/s12967-015-0697-4 |
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