T-cell-restricted intracellular antigen 1 facilitates mitochondrial fragmentation by enhancing the expression of mitochondrial fission factor
In: Cell Death & Differentiation, Jg. 24 (2016-09-09), S. 49-58
Online
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Zugriff:
Mitochondrial morphology is dynamically regulated by the formation of small fragmented units or interconnected mitochondrial networks, and this dynamic morphological change is a pivotal process in normal mitochondrial function. In the present study, we identified a novel regulator responsible for the regulation of mitochondrial dynamics. An assay using CHANG liver cells stably expressing mitochondrial-targeted yellow fluorescent protein (mtYFP) and a group of siRNAs revealed that T-cell intracellular antigen protein-1 (TIA-1) affects mitochondrial morphology by enhancing mitochondrial fission. The function of TIA-1 in mitochondrial dynamics was investigated through various biological approaches and expression analysis in human specimen. Downregulation of TIA-1-enhanced mitochondrial elongation, whereas ectopic expression of TIA-1 resulted in mitochondria fragmentation. In addition, TIA-1 increased mitochondrial activity, including the rate of ATP synthesis and oxygen consumption. Further, we identified mitochondrial fission factor (MFF) as a direct target of TIA-1, and showed that TIA-1 promotes mitochondrial fragmentation by enhancing MFF translation. TIA-1 null cells had a decreased level of MFF and less mitochondrial Drp1, a critical factor for mitochondrial fragmentation, thereby enhancing mitochondrial elongation. Taken together, our results indicate that TIA-1 is a novel factor that facilitates mitochondrial dynamics by enhancing MFF expression and contributes to mitochondrial dysfunction.
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T-cell-restricted intracellular antigen 1 facilitates mitochondrial fragmentation by enhancing the expression of mitochondrial fission factor
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Autor/in / Beteiligte Person: | Jung Woo Eun ; Kang, Hoin ; Tak, Hyosun ; Kim, Jihye ; Eun Kyung Lee ; Kyoung Bun Lee ; So Jung Park ; Kim, Chongtae ; Kim, Wook ; Ji, Eunbyul ; Lee, Heejin ; Suk Woo Nam ; Cho, Dong-Hyung |
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Zeitschrift: | Cell Death & Differentiation, Jg. 24 (2016-09-09), S. 49-58 |
Veröffentlichung: | Springer Science and Business Media LLC, 2016 |
Medientyp: | unknown |
ISSN: | 1476-5403 (print) ; 1350-9047 (print) |
DOI: | 10.1038/cdd.2016.90 |
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