Activation of muscarinic receptors in rat subfornical organ neurones
In: Journal of neuroendocrinology, Jg. 15 (2003-07-02), Heft 8
Online
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Zugriff:
Cholinergic muscarinic inputs to subfornical organ (SFO) neurones in rats were studied using histochemical, molecular-biological and electrophysiological techniques. Neurones in the medial septum and the diagonal band (MS-DBB) were retrogradely labelled by a tracer wheat germ agglutinin-conjugated horseradish peroxidase-colloidal gold complex injected into the SFO. Some in the MS-DBB were double-labelled by choline acetyltransferase (ChAT) antibody. Many ChAT-immunoreactive fibres were observed in the SFO. M3 muscarinic receptor subtype-like immunoreactivity, detected using a polyclonal antiserum, was observed in the SFO. In slice preparations, muscarine induced inward currents in a dose-related manner. The inward currents were suppressed by the relatively M3 muscarinic receptor selective antagonist 4-diphenylacetoxy-N-methylpiredine methiodide. In the whole-cell current mode, muscarine depolarized the membrane with increased frequency of action potentials. Reverse transcriptase-polymerase chain reaction showed the presence of M2-M5 receptor mRNA in the SFO tissues. These results suggest that the SFO receives cholinergic muscarinic synaptic inputs from the MS-DBB. Acetylcholine postsynaptically activates and depolarizes neurones in the SFO partly through specific muscarinic receptors, including M3 receptor subtypes.
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Activation of muscarinic receptors in rat subfornical organ neurones
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Autor/in / Beteiligte Person: | E, Honda ; K, Ono ; T, Toyono ; H, Kawano ; S, Masuko ; K, Inenaga |
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Zeitschrift: | Journal of neuroendocrinology, Jg. 15 (2003-07-02), Heft 8 |
Veröffentlichung: | 2003 |
Medientyp: | unknown |
ISSN: | 0953-8194 (print) |
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