Probenicid inhibition of fluorescence extrusion after MCB-staining of rat-1 fibroblasts
In: Cytometry, Jg. 23 (1996), Heft 1
Online
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Zugriff:
The intracellular fluorescence level of cells stained continuously with monochlorobimane was monitored by flow cytometry in order to assess the initial rate of glutatione to monochlorobimane conjugation as a measure of glutathione S-transferase activity. In addition to a rapid initial increase and a plateau level, a decline in fluorescence intensity was found upon prolonged flow cytometric monitoring. Exposure to probenicid, an inhibitor of an ATP-dependent organic anion pump, prevented this decrease. Incubation with vanadate and verapamil was without effect. Thus, extrusion of fluorescentglutathione-conjugate perturbs the proportionality between initial glutathione level and monochlorobimane-dependent fluorescence intensity. Monitoring by flow cytometry the decrease in monochlorobimane-dependent fluorescence may be useful to detect multidrug resistant cells.
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Probenicid inhibition of fluorescence extrusion after MCB-staining of rat-1 fibroblasts
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Autor/in / Beteiligte Person: | M, Poot ; F N, Hudson ; A, Grossmann ; P S, Rabinovitch ; T J, Kavanagh |
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Zeitschrift: | Cytometry, Jg. 23 (1996), Heft 1 |
Veröffentlichung: | 1996 |
Medientyp: | unknown |
ISSN: | 0196-4763 (print) |
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