[Relationship between anthocyanin synthesis and activity of phenylalanine ammonia-lyase (PAL) in callus cells of Daucus carota]
In: Planta, Jg. 117 (1974-01-18), Heft 1
Online
unknown
Zugriff:
Two celllines from Daucus carota callus were tested for phenylalanine ammonia-lyase activity. The white callus (DCw) has only 15% of the enzyme activity of the blue callus (DCb). The enzyme activity reaches a maximum value after a cultivation period of 21 days, whereas the maximum in anthocyanin content is reached after 28 days. We can demonstrate here a good correlation between one of the enzymes and the end product of the anthocyanin biosynthesis.The crude extract from DCw cells has no inhibitory effect on the enzyme from DCb-cells. Gibberellic acid A3, a component of the culture medium for DCw cells, does not act as an inhibitor on the partially purified enzyme. The procedure of purification and the characteristics of phenylalanine ammonia-lyase from the callus of Daucus carota are described.
Titel: |
[Relationship between anthocyanin synthesis and activity of phenylalanine ammonia-lyase (PAL) in callus cells of Daucus carota]
|
---|---|
Autor/in / Beteiligte Person: | U, Heinzmann ; U, Seitz |
Link: | |
Zeitschrift: | Planta, Jg. 117 (1974-01-18), Heft 1 |
Veröffentlichung: | 1974 |
Medientyp: | unknown |
ISSN: | 0032-0935 (print) |
Sonstiges: |
|