Apoptotic effects of ginsenoside Rh2 on human malignant melanoma A375-S2 cells
In: Acta pharmacologica Sinica, Jg. 23 (2002-04-05), Heft 4
Online
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Zugriff:
To study the mechanism of ginsenoside-Rh2 (G-Rh2)-induced growth inhibition of A375-S2 cells.A375-S2 cell viability and the effect of caspase inhibitors on G-Rh2-induced apoptosis were measured by crystal violet assay. Changes in cellular morphology were observed by phase-contrast microscopy. Apoptosis-specific nucleosomal DNA fragmentation was assayed by agarose gel electrophoresis. Cell cycle distribution was measured by flow cytometry.G-Rh2 inhibited the A375-S2 cell growth in concentration- and time-dependent manners. Caspase family inhibitor, z-Val-Ala-Asp-fluoromethylketone (z-VAD-fmk), caspase-3 inhibitor, z-Asp-Glu-Val-Asp-fluoromethylketone (z-DEVD-fmk), and caspase-8 inhibitor, z-Ile-Glu-Asp-fluoromethylketone (z-IETD-fmk), partially inhibited G-Rh2-induced apoptosis. But caspase-1 inhibitor, Ac-Tyr-Val-Ala-Asp-chloromethyl-ketone (Ac-YVAD-cmk), did not antagonize G-Rh2 induced-cell death.G-Rh2 suppresses the growth of A375-S2 cells in vitro by inducing apoptosis. G-Rh2-induced apoptosis is partially dependent on caspase-8 and caspase-3 pathway in A375-S2 cells. Other apoptotic pathways might be also related to the induction of apoptosis by G-Rh2.
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Apoptotic effects of ginsenoside Rh2 on human malignant melanoma A375-S2 cells
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Autor/in / Beteiligte Person: | Xiao-Fang, Fei ; Ben-Xiang, Wang ; Shinichi, Tashiro ; Tie-Jin, Li ; Ji-Sheng, Ma ; Takashi, Ikejima |
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Zeitschrift: | Acta pharmacologica Sinica, Jg. 23 (2002-04-05), Heft 4 |
Veröffentlichung: | 2002 |
Medientyp: | unknown |
ISSN: | 1671-4083 (print) |
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