S2 Fig -

(A) A schematic illustration of the experimental protocol using Tbx21 -/- mice. Naïve CD4 T cells were isolated from Tbx21 -/- (CD45.2), Tbx21 +/+ (CD45.2) and congenic C57BL/6 (CD45.1) mice and cultured under Th2 conditions. Tbx21 -/- Th2 cells were mixed together with CD45.1 Wt Th2 cells, labelled with CFSE and cultured with IL-2 in the presence of 4-OHT for the indicated number of days. (B) The Tbx21 mRNA expression relative to Hprt on Th2 cells after treatment with or without IFNγ. Error bars represent the mean ± SD. Data are representative of three independent experiments. (C) Intracellular staining of IL-4 and IFNγ production of Tbx21 +/+ or Tbx21 –/– CD4 T cells cultured for 5 days under Th1-skewing or Th2-skewing conditions is shown. (D) The MFI of CFSE on Th2 cells after cultivation for the indicated number of days. Data are representative of two independent experiments. (E) A flow cytometry analysis showing the MFI of CFSE dilution in Th1 cells after the cultivation with IFNγ or anti-IFNγ Ab for the indicated days. (PDF)