Probing the Influence of Novel Organometallic Copper(II) Complexes on Spinach PSII Photochemistry Using OJIP Fluorescence Transient Measurements
In: Biomolecules, Vol 13, Iss 1058, p 1058 (2023, Jg. 13 (2023), Heft 1058, p 1058
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Zugriff:
Modern agricultural cultivation relies heavily on genetically modified plants that survive after exposure to herbicides that kill weeds. Despite this biotechnology, there is a growing need for new sustainable, environmentally friendly, and biodegradable herbicides. We developed a novel [CuL 2 ]Br 2 complex (L = bis{4H-1,3,5-triazino[2,1-b]benzothiazole-2-amine,4-(2-imidazole) that is active on PSII by inhibiting photosynthetic oxygen evolution on the micromolar level. [CuL 2 ]Br 2 reduces the F V of PSII fluorescence. Artificial electron donors do not rescind the effect of [CuL 2 ]Br 2 . The inhibitory mechanism of [CuL 2 ]Br 2 remains unclear. To explore this mechanism, we investigated the effect of [CuL 2 ]Br 2 in the presence/absence of the well-studied inhibitor DCMU on PSII-containing membranes by OJIP Chl fluorescence transient measurements. [CuL 2 ]Br 2 has two effects on Chl fluorescence transients: (1) a substantial decrease of the Chl fluorescence intensity throughout the entire kinetics, and (2) an auxiliary “diuron-like” effect. The initial decrease dominates and is observed both with and without DCMU. In contrast, the “diuron-like” effect is small and is observed only without DCMU. We propose that [CuL 2 ]Br 2 has two binding sites for PSII with different affinities. At the high-affinity site, [CuL 2 ]Br 2 produces effects similar to PSII reaction center inhibition, while at the low-affinity site, [CuL 2 ]Br 2 produces effects identical to those of DCMU. These results are compared with other PSII-specific classes of herbicides.
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Probing the Influence of Novel Organometallic Copper(II) Complexes on Spinach PSII Photochemistry Using OJIP Fluorescence Transient Measurements
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Autor/in / Beteiligte Person: | Zharmukhamedov, Sergei K. ; Shabanova, Mehriban S. ; Huseynova, Irada M. ; Mehmet Sayım Karacan ; Karacan, Nurcan ; Akar, Hande ; Kreslavski, Vladimir D. ; Alharby, Hesham F. ; Bruce, Barry D. ; Allakhverdiev, Suleyman I. |
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Zeitschrift: | Biomolecules, Vol 13, Iss 1058, p 1058 (2023, Jg. 13 (2023), Heft 1058, p 1058 |
Veröffentlichung: | MDPI AG, 2023 |
Medientyp: | academicJournal |
ISSN: | 2218-273X (print) |
DOI: | 10.3390/biom13071058 |
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