Development of a cDNA‐AFLP derived CAPS marker co‐segregating with the powdery mildew resistance gene Mlg in barley

Powdery mildew is an important pathogen of barley in many barley‐growing areas. To identify molecular markers for the resistance gene Mlg located on chromosome 4H, a cDNA‐AFLP approach was carried out on near‐isogenic lines. Based on the identification of a differentiating fragment of 37 bp, which turned out to be part of a nucleoside diphosphate kinase as estimated by blastn , a CAPS marker has been developed, which co‐segregated with Mlg in a population of 738 F 2 ‐plants. Due to its co‐dominant inheritance, clear banding pattern and close linkage this marker is well suited for marker‐based selection procedures.