Additional file 1 of LOX overexpression programming mediates the osteoclast mechanism of low peak bone mass in female offspring rats caused by pregnant dexamethasone exposure ...
figshare, 2023
academicJournal
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Additional file 1: Fig. S1. The timetable and schematic procedure of animal treatment from gestational day (GD) 0 to postnatal week (PW) 12. Fig. S2. The experiments of the knockdown efficiency of LOX-siRNA on BMMs. (A, D): LOX mRNA expression in osteoclasts. (B, E): Representative images of LOX immunohistochemical-paraffin staining with DAPI in osteoclasts. (C, F): Quantification analyses of LOX protein mean optical density. Mean ± S.E.M., n=6 per group for mRNA expression, n=3 per group for LOX immunohistochemical-paraffin. **P<1.01 vs. NC-siRNA in vitro or CON+NC in vivo. ##P<0.01 vs. DEX+NC-siRNA in vitro or PDE+NC in vivo. DEX: dexamethasone; LOX: lysyl oxidase; NC: negative control; CON: control; PDE: prenatal dexamethasone exposure. Fig. S3. Cell viability experiments on BMMs. (A) The cytotoxic effect of DEX on BMMs at different concentrations was measured by the MTS assay. (B) The cytotoxic effect of TEM on BMMs at different concentrations was measured by the MTS assay. (C) The cytotoxic effect ...