LMT USV Toolbox, a Novel Methodological Approach to Place Mouse Ultrasonic Vocalizations in Their Behavioral Contexts—A Study in Female and Male C57BL/6J Mice and in Shank3 Mutant Females
In: Frontiers in Behavioral Neuroscience ; volume 15 ; ISSN 1662-5153, 2021
academicJournal
Zugriff:
Ultrasonic vocalizations (USVs) are used as a phenotypic marker in mouse models of neuropsychiatric disorders. Nevertheless, current methodologies still require time-consuming manual input or sound recordings clean of any background noise. We developed a method to overcome these two restraints to boost knowledge on mouse USVs. The methods are freely available and the USV analysis runs online at https://usv.pasteur.cloud . As little is currently known about usage and structure of ultrasonic vocalizations during social interactions over the long-term and in unconstrained context, we investigated mouse spontaneous communication by coupling the analysis of USVs with automatic labeling of behaviors. We continuously recorded during 3 days undisturbed interactions of same-sex pairs of C57BL/6J sexually naive males and females at 5 weeks and 3 and 7 months of age. In same-sex interactions, we observed robust differences between males and females in the amount of USVs produced, in the acoustic structure and in the contexts of emission. The context-specific acoustic variations emerged with increasing age. The emission of USVs also reflected a high level of excitement during social interactions. We finally highlighted the importance of studying long-term spontaneous communication by investigating female mice lacking Shank3 , a synaptic protein associated with autism. While the previous short-time constrained investigations could not detect USV emission abnormalities, our analysis revealed robust differences in the usage and structure of the USVs emitted by mutant mice compared to wild-type female pairs.
Titel: |
LMT USV Toolbox, a Novel Methodological Approach to Place Mouse Ultrasonic Vocalizations in Their Behavioral Contexts—A Study in Female and Male C57BL/6J Mice and in Shank3 Mutant Females
|
---|---|
Autor/in / Beteiligte Person: | de Chaumont, Fabrice ; Lemière, Nathalie ; Coqueran, Sabrina ; Bourgeron, Thomas ; Ey, Elodie |
Link: | |
Zeitschrift: | Frontiers in Behavioral Neuroscience ; volume 15 ; ISSN 1662-5153, 2021 |
Veröffentlichung: | Frontiers Media SA, 2021 |
Medientyp: | academicJournal |
DOI: | 10.3389/fnbeh.2021.735920 |
Schlagwort: |
|
Sonstiges: |
|