Coiled-Coil Antagonism Regulates Activity of Venus Flytrap-Domain-Containing Sensor Kinases of the BvgS Family
In: ISSN: 2161-2129, 2018
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academicJournal
Zugriff:
International audience ; Bordetella pertussis controls the expression of its virulence regulon through the two-component system BvgAS. BvgS is a prototype for a family of mul-tidomain sensor kinases. In BvgS, helical linkers connect periplasmic Venus flytrap (VFT) perception domains to a cytoplasmic Per-Arnt-Sim (PAS) domain and the PAS domain to the dimerization/histidine phosphotransfer (DHp) domain of the kinase. The two linkers can adopt coiled-coil structures but cannot do so simultaneously. The first linker forms a coiled coil in the kinase mode and the second in the phos-phatase mode, with the other linker in both cases showing an increase in dynamic behavior. The intervening PAS domain changes its quaternary structure between the two modes. In BvgS homologues without a PAS domain, a helical "X" linker directly connects the VFT and DHp domains. Here, we used BvgS as a platform to characterize regulation in members of the PAS-less subfamily. BvgS chimeras of homologues with natural X linkers displayed various regulation phenotypes. We identified two distinct coiled-coil registers in the N-and C-terminal portions of the X linkers. Stable coil formation in the C-terminal moiety determines the phosphatase mode, similarly to BvgS; in contrast, coil formation in the N-terminal moiety along the other register leads to the kinase mode. Thus, antagonism between two registers in the VFT-DHp linker forms the basis for activity regulation in the absence of the PAS domain. The N and C moieties of the X linker play roles similar to those played by the two independent linkers in sensor kinases with a PAS domain, providing a unified mechanism of regulation for the entire family. IMPORTANCE The whooping cough agent Bordetella pertussis uses the BvgAS sensory transduction two-component system to regulate production of its virulence factors. BvgS serves as a model for a large family of multidomain bacterial sensor ki-nases. B. pertussis is virulent when BvgS functions as a kinase and avirulent when it switches to ...
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Coiled-Coil Antagonism Regulates Activity of Venus Flytrap-Domain-Containing Sensor Kinases of the BvgS Family
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Autor/in / Beteiligte Person: | Lesne, Elodie ; Dupré, Elian ; Lensink, Marc ; Locht, Camille ; Antoine, Rudy ; Jacob-Dubuisson, Françoise ; Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL) ; Institut Pasteur de Lille ; Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS) ; Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF) ; Université de Lille-Centre National de la Recherche Scientifique (CNRS) ; E.L. received a doctoral fellowship from the Region Nord-Pas-de-Calais and INSERM. This work was supported by the Agence Nationale de la Recherche (grant number ANR-13-BSV8-0002-01 to F.J.-D.). ; We thank Eva-Maria Krammer for her initial modeling work. ; ANR-13-BSV8-0002,MECA VENUS,Mécanismes moléculaires de la transduction de signal par BvgS, un modèle de la famille de récepteurs kinases bactériens à domaines Vénus Flytrap(2013) |
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Zeitschrift: | ISSN: 2161-2129, 2018 |
Veröffentlichung: | HAL CCSD ; American Society for Microbiology, 2018 |
Medientyp: | academicJournal |
DOI: | 10.1128/mBio.02052-17 |
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