Evaluation of a saliva molecular point of care for the detection of SARS-CoV-2 in ambulatory care
In: Scientific Reports, Jg. 11 (2021), Heft 1, S. 1-8
Online
academicJournal
Zugriff:
Rapid identification of SARS-CoV-2-infected individuals is a cornerstone for the control of virus spread. The sensitivity of SARS-CoV-2 RNA detection by RT-PCR is similar in saliva and nasopharyngeal swabs. Rapid molecular point-of-care tests in saliva could facilitate, broaden and speed up the diagnosis. We conducted a prospective study in two community COVID-19 screening centers to evaluate the performances of a CE-marked RT-LAMP assay (EasyCoV) designed for the detection of SARS-CoV2 RNA from fresh saliva samples, compared to nasopharyngeal RT-PCR, to saliva RT-PCR and to nasopharyngeal antigen testing. Overall, 117 of the 1718 participants (7%) tested positive with nasopharyngeal RT-PCR. Compared to nasopharyngeal RT-PCR, the sensitivity and specificity of the RT-LAMP assay in saliva were 34% and 97%, respectively. The Ct values of nasopharyngeal RT-PCR were significantly lower in the 40 true positive subjects with saliva RT-LAMP (Ct 25.9) than in the 48 false negative subjects with saliva RT-LAMP (Ct 28.4) (p = 0.028). Considering six alternate criteria for reference tests, including saliva RT-PCR and nasopharyngeal antigen, the sensitivity of saliva RT-LAMP ranged between 27 and 44%. The detection of SARS-CoV-2 in crude saliva samples with an RT-LAMP assay had a lower sensitivity than nasopharyngeal RT-PCR, saliva RT-PCR and nasopharyngeal antigen testing. Registration number: NCT04578509.
Titel: |
Evaluation of a saliva molecular point of care for the detection of SARS-CoV-2 in ambulatory care
|
---|---|
Autor/in / Beteiligte Person: | LeGoff, Jérôme ; Kernéis, Solen ; Elie, Caroline ; Mercier-Delarue, Séverine ; Gastli, Nabil ; Choupeaux, Laure ; Fourgeaud, Jacques ; Alby, Marie-Laure ; Quentin, Pierre ; Pavie, Juliette ; Brazille, Patricia ; Marie Laure Néré ; Minier, Marine ; Gabassi, Audrey ; Leroy, Chrystel ; Parfait, Béatrice ; Tréluyer, Jean-Marc ; Delaugerre, Constance |
Link: | |
Zeitschrift: | Scientific Reports, Jg. 11 (2021), Heft 1, S. 1-8 |
Veröffentlichung: | Nature Portfolio, 2021 |
Medientyp: | academicJournal |
ISSN: | 2045-2322 (print) |
DOI: | 10.1038/s41598-021-00560-8 |
Schlagwort: |
|
Sonstiges: |
|